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. 1976 Jan;17(1):275–282. doi: 10.1128/jvi.17.1.275-282.1976

Identification of a Precursor Protein to the Major Glycoproteins of Mouse Mammary Tumor Virus

C Dickson a,1, J P Puma a, S Nandi a
PMCID: PMC515412  PMID: 173889

Abstract

Mouse mammary tumor virus-producing cultures of mouse mammary tumor cells synthesize a viral-related polypeptide of molecular weight of 73,000 (gp 73) which is rapidly labeled during a short pulse but disappears during the chase concomitantly with the appearance of label in the virion glycoproteins gp 49 and gp 37.5/33.5. The addition of the protein synthesis-inhibitor cycloheximide to the chase medium has little effect on this conversion. Treatment of the proposed precursor with α-chymotrypsin leads to the formation of a polypeptide of molecular weight 49,000, similar to the major virion glycoprotein. A comparison of tryptic digest maps of the glycoproteins involved supports the hypothesis that both the viral glycoproteins gp 49 and gp 37.5/33.5 are derived from gp 73.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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