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. 1976 Jan;17(1):291–295. doi: 10.1128/jvi.17.1.291-295.1976

In Vitro Transcription of 70S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H

Marc S Collett 1, Anthony J Faras 1
PMCID: PMC515415  PMID: 54443

Abstract

The influence of Rous sarcoma virus (RSV)-associated RNase H on the in vitro synthesis of DNA by the RSV RNA-directed DNA polymerase was determined under conditions whereby RNase H activity was selectively inhibited with NaF. Not only were we unable to detect any effect on the size, structure, or genetic complexity of the DNA product synthesized in the absence of RNase H activity, but the displacement of DNA from the 70S RNA:DNA hybrid structures was also unaffected. The suitability of 70S RNA:DNA hybrid structures synthesized in vitro to serve as a substrate for RNase H is discussed.

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Selected References

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  1. Baltimore D., Smoler D. F. Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase. J Biol Chem. 1972 Nov 25;247(22):7282–7287. [PubMed] [Google Scholar]
  2. Brewer L. C., Wells R. D. Mechanistic independence of avian myeloblastosis virus DNA polymerase and ribonuclease H. J Virol. 1974 Dec;14(6):1494–1502. doi: 10.1128/jvi.14.6.1494-1502.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collett M. S., Faras A. J. In vitro transcription of DNA from the 70S RNA of Rous sarcoma virus: identification and characterization of various size classes of DNA transcripts. J Virol. 1975 Nov;16(5):1220–1228. doi: 10.1128/jvi.16.5.1220-1228.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collett M. S., Kieras R. M., Faras A. J. Studies on the replication of reticuloendotheliosis virus: detection of viral-specific DNA sequences in infected chick cells. Virology. 1975 Jun;65(2):436–445. doi: 10.1016/0042-6822(75)90049-5. [DOI] [PubMed] [Google Scholar]
  5. Faras A. J., Taylor J. M., Levinson W. E., Goodman H. M., Bishop J. M. RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template. J Mol Biol. 1973 Sep 5;79(1):163–183. doi: 10.1016/0022-2836(73)90277-5. [DOI] [PubMed] [Google Scholar]
  6. Faras A. J., Taylor J. M., McDonnell J. P., Levinson W. E., Bishop J. M. Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus. Biochemistry. 1972 Jun 6;11(12):2334–2342. doi: 10.1021/bi00762a020. [DOI] [PubMed] [Google Scholar]
  7. Faras A., Fanshier L., Garapin A. C., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerase of Rous sarcoma virus: studies on the mechanism of double-stranded deoxyribonucleic acid synthesis. J Virol. 1971 May;7(5):539–548. doi: 10.1128/jvi.7.5.539-548.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garapin A. C., McDonnell J. P., Levinson W., Quintrell N., Fanshier L., Bishop J. M. Deoxyribonucleic acid polymerase associated with Rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product. J Virol. 1970 Nov;6(5):589–598. doi: 10.1128/jvi.6.5.589-598.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Garapin A. C., Varmus H. E., Faras A. J., Levinson W. E., Bishop J. M. RNA-directed DNA synthesis by virions of Rous sarcoma virus: further characterization of the templates and the extent of their transcription. Virology. 1973 Mar;52(1):264–274. doi: 10.1016/0042-6822(73)90414-5. [DOI] [PubMed] [Google Scholar]
  10. Gillespie D., Saxinger W. C., Gallo R. C. Information transfer in cells infected by RNA tumor viruses and extension to human neoplasia. Prog Nucleic Acid Res Mol Biol. 1975;15(0):1–108. doi: 10.1016/s0079-6603(08)60117-8. [DOI] [PubMed] [Google Scholar]
  11. Grandgenett D. P., Gerard G. F., Green M. A single subunit from avian myeloblastosis virus with both RNA-directed DNA polymerase and ribonuclease H activity. Proc Natl Acad Sci U S A. 1973 Jan;70(1):230–234. doi: 10.1073/pnas.70.1.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grandgenett D. P., Gerard G. F., Green M. Ribonuclease H: a ubiquitous activity in virions of ribonucleic acid tumor viruses. J Virol. 1972 Dec;10(6):1136–1142. doi: 10.1128/jvi.10.6.1136-1142.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Green M., Gerard G. F. RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells. Prog Nucleic Acid Res Mol Biol. 1974;14(0):187–334. [PubMed] [Google Scholar]
  14. Hung P. P. Ribonucleases of Rous sarcoma virus. Virology. 1973 Feb;51(2):287–296. doi: 10.1016/0042-6822(73)90429-7. [DOI] [PubMed] [Google Scholar]
  15. Keller W., Crouch R. Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360–3364. doi: 10.1073/pnas.69.11.3360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Leis J. P., Berkower I., Hurwitz J. Mechanism of action of ribonuclease H isolated from avian myeloblastosis virus and Escherichia coli. Proc Natl Acad Sci U S A. 1973 Feb;70(2):466–470. doi: 10.1073/pnas.70.2.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mölling K., Bolognesi D. P., Bauer H., Büsen W., Plassmann H. W., Hausen P. Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. Nat New Biol. 1971 Dec 22;234(51):240–243. doi: 10.1038/newbio234240a0. [DOI] [PubMed] [Google Scholar]
  18. Quintrell N., Fanshier L., Evans B., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerase(s) of Rous sarcoma virus: effects of virion-associated endonuclease on the enzymatic product. J Virol. 1971 Jul;8(1):17–27. doi: 10.1128/jvi.8.1.17-27.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taylor J. M., Faras A. J., Varmus H. E., Levinson W. E., Bishop J. M. Ribonucleic acid directed deoxyribonucleic acid synthesis by the purified deoxyribonucleic acid polymerase of Rous sarcoma virus. Characterization of the enzymatic product. Biochemistry. 1972 Jun 6;11(12):2343–2351. doi: 10.1021/bi00762a021. [DOI] [PubMed] [Google Scholar]
  20. Temin H. M., Baltimore D. RNA-directed DNA synthesis and RNA tumor viruses. Adv Virus Res. 1972;17:129–186. doi: 10.1016/s0065-3527(08)60749-6. [DOI] [PubMed] [Google Scholar]
  21. Varmus H. E., Levinson W. E., Bishop J. M. Extent of transcription by the RNA-dependent DNA polymerase of Rous sarcoma virus. Nat New Biol. 1971 Sep 1;233(35):19–21. doi: 10.1038/newbio233019a0. [DOI] [PubMed] [Google Scholar]
  22. Verma I. M. Studies on reverse transcriptase of RNA tumor viruses III. Properties of purified Moloney murine leukemia virus DNA polymerase and associated RNase H. J Virol. 1975 Apr;15(4):843–854. doi: 10.1128/jvi.15.4.843-854.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Verma I. M. Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide. J Virol. 1975 Jan;15(1):121–126. doi: 10.1128/jvi.15.1.121-126.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wang L. H., Duesberg P. H. DNA polymerase of murine sarcoma-leukemia virus: lack of detectable RNase H and low activity with viral RNA and natural DNA templates. J Virol. 1973 Dec;12(6):1512–1521. doi: 10.1128/jvi.12.6.1512-1521.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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