Abstract
Immunologically very closely related type C RNA viruses are endogenous to the domestic cat and to an old world primate, the baboon. In the present studies, radioimmunological techniques have been developed for detection of the 15,000 and 30,000 molecular weight (MW) polypeptides of each virus. The much more pronounced type-specific antigenic determinants of the lower MW polypeptides made it possible to readily differentiate these viruses from each other as well as from a type C virus isolate from a second baboon species. Normal rhesus monkey tissues were partially purified and shown to contain a reactivity with MW and immunological properties similar to that of the baboon virus 30,000 MW polypeptide. Despite a similar degree of purification, antigenic reactivity like that of the baboon virus 15,000 MW polypeptide was undetectable even in the brodest immunological tests available for this polypeptide. The present findings indicate that the immunological properties of two structural polypeptides of closely related viruses endogenous to primate and feline species have undergone different rates of antigenic change in the course of evolution within their respective host cell genome.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
- Benveniste R. E., Lieber M. M., Livingston D. M., Sherr C. J., Todaro G. J., Kalter S. S. Infectious C-type virus isolated from a baboon placenta. Nature. 1974 Mar 1;248(5443):17–20. doi: 10.1038/248017a0. [DOI] [PubMed] [Google Scholar]
- Benveniste R. E., Todaro G. J. Evolution of C-type viral genes: inheritance of exogenously acquired viral genes. Nature. 1974 Dec 6;252(5483):456–459. doi: 10.1038/252456a0. [DOI] [PubMed] [Google Scholar]
- Benveniste R. E., Todaro G. J. Evolution of type C viral genes: I. Nucleic acid from baboon type C virus as a measure of divergence among primate species. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4513–4518. doi: 10.1073/pnas.71.11.4513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chopra H. C., Mason M. M. A new virus in a spontaneous mammary tumor of a rhesus monkey. Cancer Res. 1970 Aug;30(8):2081–2086. [PubMed] [Google Scholar]
- Fischinger P. J., Peebles P. T., Nomura S., Haapala D. K. Isolation of RD-114-like oncornavirus from a cat cell line. J Virol. 1973 Jun;11(6):978–985. doi: 10.1128/jvi.11.6.978-985.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geering G., Aoki T., Old L. J. Shared viral antigen of mammalian leukaemia viruses. Nature. 1970 Apr 18;226(5242):265–266. doi: 10.1038/226265a0. [DOI] [PubMed] [Google Scholar]
- Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
- Goldberg R. J., Scolnick E. M., Parks W. P., Yakovleva L. A., Lapin B. A. Isolation of a primate type-C virus from a lymphomatous baboon. Int J Cancer. 1974 Dec 15;14(6):722–730. doi: 10.1002/ijc.2910140605. [DOI] [PubMed] [Google Scholar]
- Hellman A., Peebles P. T., Strickland J. E., Fowler A. K., Kalter S. S., Oroszlan K. S., Gilden R. V. Baboon virus isolate M-7 with properties similar to feline virus RD-114. J Virol. 1974 Jul;14(1):133–138. doi: 10.1128/jvi.14.1.133-138.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kalter S. S., Helmke R. J., Panigel M., Heberling R. L., Felsburg P. J., Axelrod L. R. Observations of apparent C-type particles in baboon (Papio cynocephalus) placentas. Science. 1973 Mar 30;179(4080):1332–1333. doi: 10.1126/science.179.4080.1332. [DOI] [PubMed] [Google Scholar]
- Kawakami T. G., Huff S. D., Buckley P. M., Dungworth D. L., Synder S. P., Gilden R. V. C-type virus associated with gibbon lymphosarcoma. Nat New Biol. 1972 Feb 9;235(58):170–171. doi: 10.1038/newbio235170a0. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Livingston D. M., Todaro G. J. Endogenous type C virus from a cat cell clone with properties distinct from previously described feline type C virus. Virology. 1973 May;53(1):142–151. doi: 10.1016/0042-6822(73)90473-x. [DOI] [PubMed] [Google Scholar]
- McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V. C-type virus released from cultured human rhabdomyosarcoma cells. Nat New Biol. 1972 Jan 5;235(53):3–6. doi: 10.1038/newbio235003a0. [DOI] [PubMed] [Google Scholar]
- Oroszlan S., Huebner R. J., Gilden R. V. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. doi: 10.1073/pnas.68.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parks W. P., Scolnick E. M. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. doi: 10.1073/pnas.69.7.1766. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
- Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
- Sarma P. S., Tseng J., Lee Y. K., Gilden R. V. Virus similar to RD114 virus in cat cells. Nat New Biol. 1973 Jul 11;244(132):56–59. doi: 10.1038/newbio244056a0. [DOI] [PubMed] [Google Scholar]
- Schafer W., Hunsmann G., Moennig V., Noranha F., Bolognesi D. P., Green R. W., Hüper G. Polypeptides of mammalian oncornaviruses. II Characterization of murine leukemia virus polypeptide (p 15) bearing interspecies reactivity. Virology. 1975 Jan;63(1):48–59. doi: 10.1016/0042-6822(75)90369-4. [DOI] [PubMed] [Google Scholar]
- Sherr C. J., Benveniste R. E., Todaro G. J. Type C viral expression in primate tissues. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3721–3725. doi: 10.1073/pnas.71.9.3721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherr C. J., Lieber M. M., Benveniste R. E., Todaro G. J. Endogenous baboon type C virus (M7): biochemical and immunologic characterization. Virology. 1974 Apr;58(2):492–503. doi: 10.1016/0042-6822(74)90083-x. [DOI] [PubMed] [Google Scholar]
- Sherr C. J., Todaro G. J. Primate type C virus p30 antigen in cells from humans with acute leukemia. Science. 1975 Mar 7;187(4179):855–857. doi: 10.1126/science.163489. [DOI] [PubMed] [Google Scholar]
- Sherr C. J., Todaro G. J. Type C viral antigens in man. I. Antigens related to endogenous primate virus in human tumors. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4703–4707. doi: 10.1073/pnas.71.12.4703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephenson J. R., Aaronson S. A. Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells. J Virol. 1973 Sep;12(3):564–569. doi: 10.1128/jvi.12.3.564-569.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephenson J. R., Tronick S. R., Aaronson S. A. Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-Type viruses. Virology. 1974 Mar;58(1):1–8. doi: 10.1016/0042-6822(74)90135-4. [DOI] [PubMed] [Google Scholar]
- Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strand M., August J. T. Type-C RNA virus gene expression in human tissue. J Virol. 1974 Dec;14(6):1584–1596. doi: 10.1128/jvi.14.6.1584-1596.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strand M., Wilsnack R., August J. T. Structural proteins of mammalian oncogenic RNA viruses: immunological characterization of the p15 polypeptide of Rauscher murine virus. J Virol. 1974 Dec;14(6):1575–1583. doi: 10.1128/jvi.14.6.1575-1583.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strickland J. E., Fowler A. K., Kind P. P., Hellman A., Kalter S. S., Heberling R. L., Helmke R. J. Group specific antigen and RNA-directed DNA polymerase activity in normal baboon placenta. Proc Soc Exp Biol Med. 1973 Oct 1;144(1):256–258. doi: 10.3181/00379727-144-37568. [DOI] [PubMed] [Google Scholar]
- Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
- Tronick S. R., Stephenson J. R., Aaronson S. A. Comparative immunological studies of RNA C-type viruses: radioimmunoassay for a low molecular weight polypeptide of woolly monkey leukemia virus. Virology. 1974 Feb;57(2):347–356. doi: 10.1016/0042-6822(74)90174-3. [DOI] [PubMed] [Google Scholar]
- Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]