Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Feb;17(2):402–414. doi: 10.1128/jvi.17.2.402-414.1976

DNA synthesis in polyoma virus infection. IV. Mechanism of formation of closed-circular viral DNA deficient in superhelical turns.

K Yu, W P Cheevers
PMCID: PMC515431  PMID: 176422

Abstract

A marked reduction in the rate of viral DNA synthesis is accompanied by an alteration to the superhelicity of progeny DNA in polyoma virus-infected cells in which protein synthesis has been inhibited by cycloheximide. Viral DNA molecules formed in the presence of cycloheximide consist predominantly of closed-circular monometric species (referred to as form Ic) characterized by a decreased superhelix density, corresponding to deltasigmao = 0.0195, as compared to form I DNA by propidium diiodide-cesium chloride isopycnic analysis. Form Ic is synthesized on pre-existing form I templates without the intervention of progeny form I as an intermediate. It is concluded that inhibition of protein synthesis results in the alteration of some process in the closure of daughter DNA that leads to a marked reduction of superhelical turns of progeny molecules. About two-thirds of form Ic molecules return to the form I conformation upon reversal of cycloheximide inhibition by a mechanism independent of DNA replication.

Full text

PDF
402

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourgaux P., Bourgaux-Ramoisy D. Is a specific protein responsible for the supercoiling of polyoma DNA? Nature. 1972 Jan 14;235(5333):105–107. doi: 10.1038/235105a0. [DOI] [PubMed] [Google Scholar]
  2. Bourgaux P. On the origin of oligomer forms of polyoma DNA. J Mol Biol. 1973 Jun 25;77(2):197–206. doi: 10.1016/0022-2836(73)90331-8. [DOI] [PubMed] [Google Scholar]
  3. Branton P. E., Cheevers W. P., Sheinin R. The effect of cycloheximide on DNA synthesis in cells productively-infected with polyoma virus. Virology. 1970 Dec;42(4):979–992. doi: 10.1016/0042-6822(70)90346-6. [DOI] [PubMed] [Google Scholar]
  4. Branton P. E., Sheinin R. Control of DNA synthesis in cells infected with polyoma virus. Virology. 1968 Dec;36(4):652–661. doi: 10.1016/0042-6822(68)90196-7. [DOI] [PubMed] [Google Scholar]
  5. Champoux J. J., Dulbecco R. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay). Proc Natl Acad Sci U S A. 1972 Jan;69(1):143–146. doi: 10.1073/pnas.69.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cheevers W. P., Branton P. E., Sheinin R. Characterization of abnormal DNA formed in polyoma virus-infected cells. Virology. 1970 Mar;40(3):768–772. doi: 10.1016/0042-6822(70)90227-8. [DOI] [PubMed] [Google Scholar]
  7. Cheevers W. P., Kowalski J., Yu K. K. Synthesis of high-molecular-weight cellular DNA in productive polyoma virus infection. J Mol Biol. 1972 Mar 28;65(2):347–364. doi: 10.1016/0022-2836(72)90286-0. [DOI] [PubMed] [Google Scholar]
  8. Cheevers W. P. Protein and messenger RNA requirements for superhelicity of polyoma virus DNA. Nat New Biol. 1973 Apr 18;242(120):202–204. doi: 10.1038/newbio242202a0. [DOI] [PubMed] [Google Scholar]
  9. Eason R., Vinograd J. Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid. J Virol. 1971 Jan;7(1):1–7. doi: 10.1128/jvi.7.1.1-7.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fareed G. C., McKerlie M. L., Salzman N. P. Characterization of Simian virus 40 DNA component II during viral DNA replication. J Mol Biol. 1973 Feb 25;74(2):95–111. doi: 10.1016/0022-2836(73)90101-0. [DOI] [PubMed] [Google Scholar]
  11. Hudson B., Upholt W. B., Devinny J., Vinograd J. The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA. Proc Natl Acad Sci U S A. 1969 Mar;62(3):813–820. doi: 10.1073/pnas.62.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jaenisch R., Levine A. J. DNA replication of SV40-infected cells. VII. Formation of SV40 catenated and circular dimers. J Mol Biol. 1973 Jan 10;73(2):199–212. doi: 10.1016/0022-2836(73)90323-9. [DOI] [PubMed] [Google Scholar]
  13. Keller W., Wendel I. Stepwise relaxation of supercoiled SV40 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):199–208. doi: 10.1101/sqb.1974.039.01.026. [DOI] [PubMed] [Google Scholar]
  14. Roman A., Champoux J. J., Dulbecco R. Characterization of the replicative intermediates of polyoma virus. Virology. 1974 Jan;57(1):147–160. doi: 10.1016/0042-6822(74)90116-0. [DOI] [PubMed] [Google Scholar]
  15. STANNERS C. P. STUDIES ON THE TRANSFORMATION OF HAMSTER EMBRYO CELLS IN CULTURE BY POLYOMA VIRUS. II. SELECTIVE TECHNIQUES FOR THE DETECTION OF TRANSFORMED CELLS. Virology. 1963 Nov;21:464–476. doi: 10.1016/0042-6822(63)90207-1. [DOI] [PubMed] [Google Scholar]
  16. Sen A., Levine A. J. SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNA. Nature. 1974 May 24;249(455):343–344. doi: 10.1038/249343a0. [DOI] [PubMed] [Google Scholar]
  17. Vinograd J., Lebowitz J., Watson R. Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA. J Mol Biol. 1968 Apr 14;33(1):173–197. doi: 10.1016/0022-2836(68)90287-8. [DOI] [PubMed] [Google Scholar]
  18. Wang J. C. Interaction between DNA and an Escherichia coli protein omega. J Mol Biol. 1971 Feb 14;55(3):523–533. doi: 10.1016/0022-2836(71)90334-2. [DOI] [PubMed] [Google Scholar]
  19. White M., Eason R. Supercoiling of SV40 DNA can occur independently of replication. Nat New Biol. 1973 Jan 10;241(106):46–49. doi: 10.1038/newbio241046a0. [DOI] [PubMed] [Google Scholar]
  20. Yu K., Cheevers W. P. DNA synthesis in polyoma virus infection. V. Kinetic evidence for two requirements for protein synthesis during viral DNA replication. J Virol. 1976 Feb;17(2):415–421. doi: 10.1128/jvi.17.2.415-421.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yu K., Kowalski J., Cheevers W. DNA synthesis in polyoma virus infection. III. Mechanism of inhibition of viral DNA replication by cycloheximide. J Virol. 1975 Jun;15(6):1409–1417. doi: 10.1128/jvi.15.6.1409-1417.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES