Abstract
When cultures producing reticuloendotheliosis virus were incubated for 24 h in medium of lowered NaCl concentration, virus production was inhibited. The extent of inhibition increased as the salt concentration of the medium was decreased. The inhibition was rapidly reversed by replacement of low-salt medium with normal medium. During the first hour after the inhibited cultures were returned to normal medium, virus was released at an accelerated rate, making the total amount of virus released by inhibited and control cultures the same. After 1 h in normal medium, the rate of virus production in the previously inhibited cultures was the same as in the control cultures. Incubation of infected cells in low-salt medium resulted in a 60% decrease in the overall rate of protein synthesis. Although returning the cells to normal medium rapidly reversed the inhibition of virus production, it did not rapidly increase the rate of protein synthesis. These results suggest that host cell-directed protein synthesis is preferentially inhibited by the low-ionic-strength medium, whereas that required for virus production continues.
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- Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bose H. R., Jr, Levine A. S. Replication of the reticuloendotheliosis virus (strain T) in chicken embryo cell culture. J Virol. 1967 Dec;1(6):1117–1121. doi: 10.1128/jvi.1.6.1117-1121.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EATON M. D., SCALA A. R. Reversible effect of hypotonic solutions on growth of influenza virus in tissue cultures. Proc Soc Exp Biol Med. 1956 Jun;92(2):289–297. doi: 10.3181/00379727-92-22454. [DOI] [PubMed] [Google Scholar]
- Maldonado R. L., Bose H. R. Separation of reticuloendotheliosis virus from avian tumor viruses. J Virol. 1971 Nov;8(5):813–815. doi: 10.1128/jvi.8.5.813-815.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Purchase H. G., Ludford C., Nazerian K., Cox H. W. A new group of oncogenic viruses: reticuloendotheliosis, chick syncytial, duck infectious anemia, and spleen necrosis viruses. J Natl Cancer Inst. 1973 Aug;51(2):489–499. [PubMed] [Google Scholar]
- Shanmugam G., Vecchio G., Attardi D., Green M. Immunological studies on viral polypeptide synthesis in cells replicating murine sarcoma-leukemia virus. J Virol. 1972 Sep;10(3):447–455. doi: 10.1128/jvi.10.3.447-455.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TEMIN H. M. THE EFFECTS OF ACTINOMYCIN D ON GROWTH OF ROUS SARCOMA VIRUS IN VITRO. Virology. 1963 Aug;20:577–582. doi: 10.1016/0042-6822(63)90282-4. [DOI] [PubMed] [Google Scholar]
- Temin H. M., Kassner V. K. Replication of reticuloendotheliosis viruses in cell culture: acute infection. J Virol. 1974 Feb;13(2):291–297. doi: 10.1128/jvi.13.2.291-297.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waite M. R., Allen P. T. RNA-directed DNA polymerase activity of reticuloendotheliosis virus: characterization of the endogenous and exogenous reactions. J Virol. 1975 Oct;16(4):872–879. doi: 10.1128/jvi.16.4.872-879.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waite M. R., Brown D. T., Pfefferkorn E. R. Inhibition of Sindbis virus release by media of low ionic strength: an electron microscope study. J Virol. 1972 Sep;10(3):537–544. doi: 10.1128/jvi.10.3.537-544.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waite M. R., Pfefferkorn E. R. Effect of altered osmotic pressure on the growth of Sindbis virus. J Virol. 1968 Jul;2(7):759–760. doi: 10.1128/jvi.2.7.759-760.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waite M. R., Pfefferkorn E. R. Inhibition of Sindbis virus production by media of low ionic strength: intracellular events and requirements for reversal. J Virol. 1970 Jan;5(1):60–71. doi: 10.1128/jvi.5.1.60-71.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]