Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Feb;17(2):525–537. doi: 10.1128/jvi.17.2.525-537.1976

Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses.

K L Beemon, A J Faras, A T Hasse, P H Duesberg, J E Maisel
PMCID: PMC515444  PMID: 176429

Abstract

The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1-resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5 x 10(6) daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3 x 10(6). Reticuleondotheliosis and visna virus RNAs had complexities of 3.9 x 10(6), respectively. Analysis of RNase A-resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6 x 10(6), similar to that previously obtained with RNase T1-resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA subunit, it was concluded that ribodeoxyvirus genomes are at least largely polyploid.

Full text

PDF
525

Images in this article

527Image
on p.527
530Image
on p.530
532Image
on p.532
534Image
on p.534

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball J., McCarter J. A., Sunderland S. M. Evidence for helper independent murine sarcoma virus. I. Segregation of replication-defective and transformation-defective viruses. Virology. 1973 Nov;56(1):268–284. doi: 10.1016/0042-6822(73)90305-x. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D. Tumor viruses: 1974. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1187–1200. doi: 10.1101/sqb.1974.039.01.137. [DOI] [PubMed] [Google Scholar]
  3. Baluda M. A., Shoyab M., Markham P. D., Evans R. M., Droham W. N. Base sequence complexity of 35S avian myeloblastosis virus RNA determined by molecular hybridization kinetics. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):869–874. doi: 10.1101/sqb.1974.039.01.101. [DOI] [PubMed] [Google Scholar]
  4. Beemon K., Duesberg P., Vogt P. Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254–4258. doi: 10.1073/pnas.71.10.4254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Billeter M. A., Parsons J. T., Coffin J. M. The nucleotide sequence complexity of avian tumor virus RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560–3564. doi: 10.1073/pnas.71.9.3560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Blair C. D., Duesberg P. H. Structure of Rauscher mouse leukaemia virus RNA. Nature. 1968 Oct 26;220(5165):396–399. doi: 10.1038/220396a0. [DOI] [PubMed] [Google Scholar]
  7. Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
  8. Cooper G. M., Temin H. M. Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. J Virol. 1974 Nov;14(5):1132–1141. doi: 10.1128/jvi.14.5.1132-1141.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cooper P. D., Wyke J. A. The genome of RNA tumor viruses: a functional requirement for a polyploid structure? Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):997–1004. doi: 10.1101/sqb.1974.039.01.114. [DOI] [PubMed] [Google Scholar]
  10. Dahlberg J. E., Sawyer R. C., Taylor J. M., Faras A. J., Levinson W. E., Goodman H. M., Bishop J. M. Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer. J Virol. 1974 May;13(5):1126–1133. doi: 10.1128/jvi.13.5.1126-1133.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Delius H., Duesberg P. H., Mangel W. F. Electron microscope measurements of rous sarcoma virus RNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):835–843. doi: 10.1101/sqb.1974.039.01.097. [DOI] [PubMed] [Google Scholar]
  12. Duesberg P. H., Vogt P. K. Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species. J Virol. 1973 Sep;12(3):594–599. doi: 10.1128/jvi.12.3.594-599.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Duesberg P. H., Vogt P. K. RNA species obtained from clonal lines of avian sarcoma and from avian leukosis virus. Virology. 1973 Jul;54(1):207–219. doi: 10.1016/0042-6822(73)90130-x. [DOI] [PubMed] [Google Scholar]
  14. Duesberg P., Vogt P. K., Beemon K., Lai M. Avian RNA tumor viruses: mechanism of recombination and complexity of the genome. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):847–857. doi: 10.1101/sqb.1974.039.01.099. [DOI] [PubMed] [Google Scholar]
  15. Erikson E., Erikson R. L. Association of 4S ribonucleic acid with oncornavirus ribonucleic acids. J Virol. 1971 Aug;8(2):254–256. doi: 10.1128/jvi.8.2.254-256.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fan H., Paskind M. Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome. J Virol. 1974 Sep;14(3):421–429. doi: 10.1128/jvi.14.3.421-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Fiers W., Lepoutre L., Vandendriessche L. Studies on the bacteriophage MS2. I. Distribution of purine sequences in the viral RNA and in yeast RNA. J Mol Biol. 1965 Sep;13(2):432–450. doi: 10.1016/s0022-2836(65)80108-5. [DOI] [PubMed] [Google Scholar]
  18. Gibson W., Verma I. M. Studies on the reverse transcriptase of RNA tumor viruses. Structural relatedness of two subunits of avian RNA tumor viruses. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4991–4994. doi: 10.1073/pnas.71.12.4991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Haase A. T., Baringer J. R. The structural polypeptides of RNA slow viruses. Virology. 1974 Jan;57(1):238–250. doi: 10.1016/0042-6822(74)90124-x. [DOI] [PubMed] [Google Scholar]
  20. Haase A. T., Garapin A. C., Faras A. J., Taylor J. M., Bishop J. M. A comparison of the high molecular weight RNAs of visna virus and Rous sarcoma virus. Virology. 1974 Jan;57(1):259–270. doi: 10.1016/0042-6822(74)90126-3. [DOI] [PubMed] [Google Scholar]
  21. Halpern M. S., Wade E., Rucker E., Baxter-Gabbard K. L., Levine A. S., Friis R. R. A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses. Virology. 1973 Jun;53(2):287–299. doi: 10.1016/0042-6822(73)90206-7. [DOI] [PubMed] [Google Scholar]
  22. Ihle J. N., Lee K. L., Kennedy F. T. Fractionation of 34 S ribonucleic acid subunits from oncornaviruses on polyuridylate-sepharose columns. J Biol Chem. 1974 Jan 10;249(1):38–42. [PubMed] [Google Scholar]
  23. Kieras R. M., Faras A. J. DNA polymerase of reticuloendotheliosis virus: inability to detect endogenous RNA-directed DNA synthesis. Virology. 1975 Jun;65(2):514–523. doi: 10.1016/0042-6822(75)90056-2. [DOI] [PubMed] [Google Scholar]
  24. Kung H. J., Bailey J. M., Davidson N., Nicolson M. O., McAllister R. M. Structure, subunit composition, and molecular weight of RD-114 RNA. J Virol. 1975 Aug;16(2):397–411. doi: 10.1128/jvi.16.2.397-411.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kung H. J., Bailey J. M., Davidson N., Vogt P. K., Nicolson M. O., McAllister R. M. Electron microscope studies of tumor virus RNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):827–834. doi: 10.1101/sqb.1974.039.01.096. [DOI] [PubMed] [Google Scholar]
  26. Lo A. C., Ball J. K. Evidence for helper-independent murine sarcoma virus. II. Differences between the ribonucleic acids of clone-purified leukemia virus, helper-independent and helper-dependent sarcoma viruses. Virology. 1974 Jun;59(2):545–555. doi: 10.1016/0042-6822(74)90464-4. [DOI] [PubMed] [Google Scholar]
  27. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  28. Maisel J., Klement V., Lai M. M., Ostertag W., Duesberg P. Ribonucleic acid components of murine sarcoma and leukemia viruses. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3536–3540. doi: 10.1073/pnas.70.12.3536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Maisel J., Scolnick E. M., Duesberg P. Base sequence differences between the RNA components of Harvey sarcoma virus. J Virol. 1975 Sep;16(3):749–753. doi: 10.1128/jvi.16.3.749-753.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mangel W. F., Delius H., Duesberg P. H. Structure and molecular weight of the 60-70S RNA and the 30-40S RNA of the Rous sarcoma virus. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4541–4545. doi: 10.1073/pnas.71.11.4541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Miller M. F., Allen P. T., Dmochowski L. Quantitative studies of oncornaviruses in thin sections. J Gen Virol. 1973 Oct;21:57–68. doi: 10.1099/0022-1317-21-1-57. [DOI] [PubMed] [Google Scholar]
  32. Moelling K. Reverse transcriptase and RNase H: present in a murine virus and in both subunits of an avian virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):969–973. doi: 10.1101/sqb.1974.039.01.111. [DOI] [PubMed] [Google Scholar]
  33. Quade K., Smith R. E., Nichols J. L. Evidence for common nucleotide sequences in the RNA subunits comprising Rous sarcoma virus 70 S RNA. Virology. 1974 Sep;61(1):287–291. doi: 10.1016/0042-6822(74)90263-3. [DOI] [PubMed] [Google Scholar]
  34. Rho H. M., Grandgenett D. P., Green M. Sequence relatedness between the subunits of avian myeloblastosis virus reverse transcriptase. J Biol Chem. 1975 Jul 10;250(13):5278–5280. [PubMed] [Google Scholar]
  35. SINHA N. K., FUJIMURA R. K., KAESBERG P. RIBONUCLEASE DIGESTION OF R17 VIRAL RNA. J Mol Biol. 1965 Jan;11:84–89. doi: 10.1016/s0022-2836(65)80173-5. [DOI] [PubMed] [Google Scholar]
  36. Straus N. A., Bonner T. I. Temperature dependence of RNA-DNA hybridization kinetics. Biochim Biophys Acta. 1972 Aug 16;277(1):87–95. doi: 10.1016/0005-2787(72)90355-3. [DOI] [PubMed] [Google Scholar]
  37. Taylor J. M., Varmus H. E., Faras A. J., Levinson W. E., Bishop J. M. Letter: Evidence for non-repetitive subunits in the genome of Rous sarcoma virus. J Mol Biol. 1974 Mar 25;84(1):217–221. doi: 10.1016/0022-2836(74)90223-x. [DOI] [PubMed] [Google Scholar]
  38. Wang L. H., Duesberg P., Beemon K., Vogt P. K. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end. J Virol. 1975 Oct;16(4):1051–1070. doi: 10.1128/jvi.16.4.1051-1070.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wang L. H., Duesberg P. Properties and location of poly(A) in Rous sarcoma virus RNA. J Virol. 1974 Dec;14(6):1515–1529. doi: 10.1128/jvi.14.6.1515-1529.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Weissmann C., Parsons J. T., Coffin J. W., Rymo L., Billeter M. A., Hofstetter H. Studies on the structure and synthesis of Rous sarcoma virus RNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1043–1056. doi: 10.1101/sqb.1974.039.01.120. [DOI] [PubMed] [Google Scholar]
  41. Wyke J. A., Bell J. G., Beamand J. A. Genetic recombination among temperature-sensitive mutnats of Rous sarcoma virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):897–905. doi: 10.1101/sqb.1974.039.01.104. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES