Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Feb;17(2):597–604. doi: 10.1128/jvi.17.2.597-604.1976

High-titer replication of nondefective Sendai virus in MDBK cells.

N G Famulari, E Fleissner
PMCID: PMC515450  PMID: 176432

Abstract

Egg-grown Sendai virus was adapted to growth in a bovine kidney cell line (MDBK cells) by serial passage under defined conditions. The adapted virus contained only 50S RNA and was highly infectious for MDBK cells. Infection of these cells with a high multiplicity of adapted virus resulted in a yield of 10(8) MDBK-infectious units/ml by 18 h, accompanied by severe cytopathic changes in the host. Cell fusion did not occur. Examination of the proteins of the adapted virus revealed that despite the high infectivity of this virus for MDBK cells the virions contained considerable quantities of Fo, the precursor to the F glycoprotein that is responsible for cell fusion and high infectivity in other systems.

Full text

PDF
597

Images in this article

600Image
on p.600
602Image
on p.602

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baum S. G., Horwitz M. S., Maizel J. V., Jr Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. J Virol. 1972 Aug;10(2):211–219. doi: 10.1128/jvi.10.2.211-219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blair C. D., Robinson W. S. Replication of Sendai virus. II. Steps in virus assembly. J Virol. 1970 May;5(5):639–650. doi: 10.1128/jvi.5.5.639-650.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHOPPIN P. W. MULTIPLICATION OF TWO KINDS OF INFLUENZA A2 VIRUS PARTICLES IN MONKEY KIDNEY CELLS. Virology. 1963 Nov;21:342–352. doi: 10.1016/0042-6822(63)90195-8. [DOI] [PubMed] [Google Scholar]
  4. Choppin P. W. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969 Sep;39(1):130–134. doi: 10.1016/0042-6822(69)90354-7. [DOI] [PubMed] [Google Scholar]
  5. Darlington R. W., Portner A., Kingsbury D. W. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells. J Gen Virol. 1970 Dec;9(3):169–177. doi: 10.1099/0022-1317-9-3-169. [DOI] [PubMed] [Google Scholar]
  6. Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes K. V., Choppin P. W. On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types. J Exp Med. 1966 Sep 1;124(3):501–520. doi: 10.1084/jem.124.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Homma M., Ouchi M. Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells. J Virol. 1973 Dec;12(6):1457–1465. doi: 10.1128/jvi.12.6.1457-1465.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Homma M. Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of tyrpsin on L cell-borne Sendai virus. J Virol. 1971 Nov;8(5):619–629. doi: 10.1128/jvi.8.5.619-629.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ikeda H., Hardy W., Jr, Tress E., Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. V. Identification of a new murine viral protein, p15(E). J Virol. 1975 Jul;16(1):53–61. doi: 10.1128/jvi.16.1.53-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
  12. Kingsbury D. W., Portner A. On the genesis of incomplete Sendai virions. Virology. 1970 Dec;42(4):872–879. doi: 10.1016/0042-6822(70)90336-3. [DOI] [PubMed] [Google Scholar]
  13. Klenk H. D., Choppin P. W. Plasma membrane lipids and parainfluenza virus assembly. Virology. 1970 Apr;40(4):939–947. doi: 10.1016/0042-6822(70)90140-6. [DOI] [PubMed] [Google Scholar]
  14. Krug R. M. Influenza viral RNPs newly synthesized during the latent period of viral growth in MDCK cells. Virology. 1971 Apr;44(1):125–136. doi: 10.1016/0042-6822(71)90159-0. [DOI] [PubMed] [Google Scholar]
  15. Lazarowitz S. G., Compans R. W., Choppin P. W. Proteolytic cleavage of the hemagglutinin polypeptide of influenza virus. Function of the uncleaved polypeptide HA. Virology. 1973 Mar;52(1):199–212. doi: 10.1016/0042-6822(73)90409-1. [DOI] [PubMed] [Google Scholar]
  16. MATSUMOTO T., YAMAMOTO N., MAENO K. Effect of egg passage on the reaction between HVJ (hemagglutinating virus of Japan) and chicken erythrocytes. J Immunol. 1961 Nov;87:590–598. [PubMed] [Google Scholar]
  17. NORTHROP R. L., WALKER D. L. A STABLE HUMAN CELL LINE AS A HOST SYSTEM FOR SENDAI VIRUS. Proc Soc Exp Biol Med. 1965 Mar;118:698–703. doi: 10.3181/00379727-118-29943. [DOI] [PubMed] [Google Scholar]
  18. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
  19. Wickus G. G., Robbins P. W. Plasma membrane proteins of normal and Rous sarcoma virus-transformed chick-embryo fibroblasts. Nat New Biol. 1973 Sep 19;245(142):65–67. doi: 10.1038/newbio245065a0. [DOI] [PubMed] [Google Scholar]
  20. ZHDANOV V. M., BUKRINSKAYA A. G., AZADOVA N. B. Fluorescent microscopic study of Sendai incomplete virus production in tissue culture cells. J Immunol. 1961 Dec;87:647–653. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES