Abstract
T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a synthesis of T antigen on cytoplasmic ribosomes, with subsequent transfer to the nucleus.
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- Baba T., Yamaguchi N., Ishida R., Suzuki I. Ultrastructural localization of T-antigen in SV40-transformed kidney cells of a green monkey by the pepoxidase-labeled antibody method. Gan. 1973 Apr;64(2):173–177. [PubMed] [Google Scholar]
- Brugge J. S., Butel J. S. Role of simian virus 40 gene A function in maintenance of transformation. J Virol. 1975 Mar;15(3):619–635. doi: 10.1128/jvi.15.3.619-635.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOGGAN M. D., ROWE W. P., BLACK P. H., HUEBNER R. J. PRODUCTION OF "TUMOR-SPECIFIC" ANTIGENS BY ONCOGENIC VIRUSES DURING ACUTE CYTOLYTIC INFECTIONS. Proc Natl Acad Sci U S A. 1965 Jan;53:12–19. doi: 10.1073/pnas.53.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kraehenbuhl J. P., De Grandi P. B., Campiche M. A. Ultrastructural localization of intracellular antigen using enzyme-labeled antibody fragments. J Cell Biol. 1971 Aug;50(2):432–445. doi: 10.1083/jcb.50.2.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leduc E. H., Wicker R., Avrameas S., Bernhard W. Ultrastructural localization of SV40T antigen with enzyme-labelled antibody. J Gen Virol. 1969 Jun;4(4):609–614. doi: 10.1099/0022-1317-4-4-609. [DOI] [PubMed] [Google Scholar]
- Lehman J. M., Defendi V. Changes in deoxyribonucleic acid synthesis regulation in Chinese hamster cells infected with simian virus 40. J Virol. 1970 Dec;6(6):738–749. doi: 10.1128/jvi.6.6.738-749.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lehman J. M., Defendi V. Induction of fibrosarcomas in rabbits by polyoma virus. J Natl Cancer Inst. 1970 Jan;44(1):125–132. [PubMed] [Google Scholar]
- Martin R. G., Chou J. Y. Simian virus 40 functions required for the establishment and maintenance of malignant transformation. J Virol. 1975 Mar;15(3):599–612. doi: 10.1128/jvi.15.3.599-612.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller M. H., Karnovsky M. J., Diamandopoulos G. T. An improved immunoperoxidase technique for identifying SV40 V and T antigens by light microscopy. Proc Soc Exp Biol Med. 1974 Jun;146(2):432–437. doi: 10.3181/00379727-146-38120. [DOI] [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- Oshiro L. S., Rose H. M., Morgan C., Hsu K. C. The localization of SV40-induced neoantigen with ferritin-labeled antibody. Virology. 1967 Jan;31(1):183–186. doi: 10.1016/0042-6822(67)90028-1. [DOI] [PubMed] [Google Scholar]
- POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tegtmeyer P. Function of simian virus 40 gene A in transforming infection. J Virol. 1975 Mar;15(3):613–618. doi: 10.1128/jvi.15.3.613-618.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]