In Table 2, the IonA Forward primer sequence is listed incorrectly. Please see the corrected Table 2 here.
Table 2. Primers used for Sanger sequencing, NGS and in IAC.
| Region/Primer name | Direction | Primer sequence | Reference |
|---|---|---|---|
| rbcL | |||
| rbcLa-F | Forward | ATGTCACCACAAACAGAGACTAAAGC | [57] |
| rbcLa-R | Reverse | GTAAAATCAAGTCCACCRCG | [58] |
| MrbcL 163-R1 | Reverse | CGGTCCAYACAGYBGTCCAKGTACC | this study |
| IAC | |||
| LepF1 | Forward | ATTCAACCAATCATAAAGATATTGG | [55] |
| LepR1 | Reverse | TAAACTTCTGGATGTCCAAAAAATCA | [55] |
| ITS2 | |||
| ITS3 | Forward | GCATCGATGAAGAACGCAGC | [59] |
| ITS_S2F | Forward | ATGCGATACTTGGTGTGAAT | [60] |
| ITS-S2F-GINK | Forward | ATGCGATATTTAGTGTGAAT | this study |
| ITS4 | Reverse | TCCTCCGCTTATTGATATGC | [59] |
| NGS-fusion | |||
| IonA | Forward | CCATCTCATCCCTGCGTGTCTCCGAC [TCAG][IonExpress-MID][specific sequence] | Ion Torrent, Thermo Fisher Scientific |
| trP1 | Reverse | CCTCTCTATGGGCAGTCGGTGAT[specific sequence] | Ion Torrent, Thermo Fisher Scientific |
Reference
- 1.Ivanova NV, Kuzmina ML, Braukmann TWA, Borisenko AV, Zakharov EV (2016) Authentication of Herbal Supplements Using Next-Generation Sequencing. PLoS ONE 11(5): e0156426 doi: 10.1371/journal.pone.0156426 [DOI] [PMC free article] [PubMed] [Google Scholar]
