Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Mar;17(3):885–893. doi: 10.1128/jvi.17.3.885-893.1976

Demonstration of subpopulations of Dane particles.

P M Kaplan, E C Ford, R H Purcell, J L Gerin
PMCID: PMC515489  PMID: 1255863

Abstract

Two populations of Dane particles were isolated from the plasma of individuals carrying hepatitis B surface antigen. These populations had densities in CsCl of 1.22 and 1.20 g/ml. Endogenous DNA polymerase activity was found to be associated only with the heavier of these two populations. Using a positive stain, electron microscopic examination of these particles suggested that the heavier the particle contained nucleic acid in its core whereas the lighter particle appeared empty. Cores isolated from Dane particles with densities of 1.22 and 1.20 g/ml banded in CsCl at densities of 1.36 and 1.30 g/ml, respectively. Endogenous DNA polymerase activity was associated only with the higher density core particles.

Full text

PDF
885

Images in this article

890Image
on p.890

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D. Electron microscopic observations and speculations on Australia antigen. Postgrad Med J. 1971 Jul;47(549):484–487. doi: 10.1136/pgmj.47.549.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker L. F., Almeida J. D., Hoofnagle J. H., Gerety R. J., Jackson D. R., McGrath P. P. Hepatitis B core antigen: immunology and electron microscopy. J Virol. 1974 Dec;14(6):1552–1558. doi: 10.1128/jvi.14.6.1552-1558.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bayer M. E., Blumberg B. S., Werner B. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature. 1968 Jun 15;218(5146):1057–1059. doi: 10.1038/2181057a0. [DOI] [PubMed] [Google Scholar]
  4. Brockman W. W., Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):942–946. doi: 10.1073/pnas.71.3.942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dane D. S., Cameron C. H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970 Apr 4;1(7649):695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
  6. Gerin J. L., Holland P. V., Purcell R. H. Australia antigen: large-scale purification from human serum and biochemical studies of its proteins. J Virol. 1971 May;7(5):569–576. doi: 10.1128/jvi.7.5.569-576.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerin J. L., Purcell R. H., Hoggan M. D., Holland P. V., Chanock R. M. Biophysical properties of Australia antigen. J Virol. 1969 Nov;4(5):763–768. doi: 10.1128/jvi.4.5.763-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grady L., Axelrod D., Trilling D. The SV40 pseudovirus: its potential for general transduction in animal cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1886–1893. doi: 10.1073/pnas.67.4.1886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HALPEREN S., EGGERS H. J., TAMM I. COMPLETE AND CORELESS HEMAGGLUTINATING PARTICLES PRODUCED IN ECHO 12 VIRUS-INFECTED CELLS. Virology. 1964 May;23:81–89. doi: 10.1016/s0042-6822(64)80010-6. [DOI] [PubMed] [Google Scholar]
  10. Hirschman S. Z., Gerber M., Garfinkel E. Purification of naked intranuclear particles from human liver infected by hepatitis B virus. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3345–3349. doi: 10.1073/pnas.71.9.3345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoofnagle J. H., Gerety R. J., Barker L. F. Antibody to hepatitis-B-virus core in man. Lancet. 1973 Oct 20;2(7834):869–873. doi: 10.1016/s0140-6736(73)92004-7. [DOI] [PubMed] [Google Scholar]
  12. Jacobson M. F., Baltimore D. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein. J Mol Biol. 1968 Apr 28;33(2):369–378. doi: 10.1016/0022-2836(68)90195-2. [DOI] [PubMed] [Google Scholar]
  13. Jokelainen P. T., Krohn K., Prince A. M., Finlayson N. D. Electrn microscopic observations on virus-like particles associated with SH antigen. J Virol. 1970 Nov;6(5):685–689. doi: 10.1128/jvi.6.5.685-689.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaplan P. M., Gerin J. L., Alter H. J. Hepatitis B-specific DNA polymerase activity during post-transfusion hepatitis. Nature. 1974 Jun 21;249(459):762–764. doi: 10.1038/249762a0. [DOI] [PubMed] [Google Scholar]
  15. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Khoury G., Fareed G. C., Berry K., Martin M. A., Lee T. N., Nathans D. Characterization of a rearrangement in viral DNA: mapping of the circular simian virus 40-like DNA containing a triplication of a specific one-third of the viral genome. J Mol Biol. 1974 Aug 5;87(2):289–301. doi: 10.1016/0022-2836(74)90150-8. [DOI] [PubMed] [Google Scholar]
  17. Krugman S., Hoofnagle J. H., Gerety R. J., Kaplan P. M., Gerin J. L. Viral hepatitis, type B, DNA polymerase activity and antibody to hepatitis B core antigen. N Engl J Med. 1974 Jun 13;290(24):1331–1335. doi: 10.1056/NEJM197406132902401. [DOI] [PubMed] [Google Scholar]
  18. Lai C. J., Nathans D. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J Mol Biol. 1974 Oct 15;89(1):179–193. doi: 10.1016/0022-2836(74)90169-7. [DOI] [PubMed] [Google Scholar]
  19. Ling C. M., Overby L. R. Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody. J Immunol. 1972 Oct;109(4):834–841. [PubMed] [Google Scholar]
  20. Moritsugu Y., Gold J. W., Wagner J., Dodd R. Y., Purcell R. H. Hepatitis B core antigen. Detection of antibody by radioimmunoprecipitation. J Immunol. 1975 Jun;114(6):1792–1798. [PubMed] [Google Scholar]
  21. News. From the National Academy of Sciences: Nomenclature of antigens associated with viral hepatitis type B. J Infect Dis. 1974 Jul;130(1):92–92. doi: 10.1093/infdis/130.1.92. [DOI] [PubMed] [Google Scholar]
  22. Parks W. P., Melnick J. L., Rongey R., Mayor H. D. Physical assay and growth cycle studies of a defective adeno-satellite virus. J Virol. 1967 Feb;1(1):171–180. doi: 10.1128/jvi.1.1.171-180.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Purcell R. H., Gerin J. L., Almeida J. B., Holland P. V. Radioimmunoassay for the detection of the core of the Dane particle and antibody to it. Intervirology. 1974;2(4):231–243. doi: 10.1159/000149428. [DOI] [PubMed] [Google Scholar]
  24. Robinson W. S., Clayton D. A., Greenman R. L. DNA of a human hepatitis B virus candidate. J Virol. 1974 Aug;14(2):384–391. doi: 10.1128/jvi.14.2.384-391.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Robinson W. S., Greenman R. L. DNA polymerase in the core of the human hepatitis B virus candidate. J Virol. 1974 Jun;13(6):1231–1236. doi: 10.1128/jvi.13.6.1231-1236.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES