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. 1976 Apr;18(1):218–226. doi: 10.1128/jvi.18.1.218-226.1976

Process of infection with bacteriophage phi chi 174. XL. Viral DNA replication of phi chi 174 mutants blocked in progeny single-stranded DNA synthesis.

A Fukuda, R L Sinsheimer
PMCID: PMC515541  PMID: 1255871

Abstract

Mutation in several different cistrons of bacteriophage phi chi 174 blocks net progeny single-stranded DNA synthesis at the late period of infection (15). For the study of the functions of these cistrons in single-stranded DNA synthesis, asymmetric replication of replicative form DNA was examined at the late period of infection with amber mutants of these cistrons. While the normal, rapid process of asymmetric single-stranded viral DNA synthesis is blocked at the late period of these mutant infections, an asymmetric synthesis of the viral strand of replicative-form DNA is observed in this period, though at a reduced level, together with degradation of prelabeled viral strand. Some intermediate replicative-form molecules were also detected. Asymmetric synthesis of the viral strand of replicative-form DNA at the late period of phi chi infection is completely inhibited in the presence of a low concentration (35mug/ml) of chloramphenicol (which also blocks net single-stranded viral DNA synthesis). These results are discussed in terms of the possible role of the specific viral proteins for normal single-stranded DNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Vinogradj The interaction of closed circular DNA with intercalative dyes. 3. Dependence of the buoyant density upon superhelix density and base composition. J Mol Biol. 1970 Dec 14;54(2):281–298. doi: 10.1016/0022-2836(70)90430-4. [DOI] [PubMed] [Google Scholar]
  2. Bowman K. L., Ray D. S. Degradation of the viral strand of phiX174 parental replicative-form DNA in a rep- host. J Virol. 1975 Oct;16(4):838–843. doi: 10.1128/jvi.16.4.838-843.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dressler D. The rolling circle for phiX DNA replication. II. Synthesis of single-stranded circles. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1934–1942. doi: 10.1073/pnas.67.4.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dumas L. B., Darby G., Sinsheimer R. L. The replication of bacteriophage phi X174 DNA in vitro. Temperature effects on repair synthesis and displacement synthesis. Biochim Biophys Acta. 1971 Jan 28;228(2):407–422. [PubMed] [Google Scholar]
  5. Funk F. D., Sinsheimer R. L. Process of infection with bacteriophage phiX174. XXXV. Cistron 8. J Virol. 1970 Jul;6(1):12–19. doi: 10.1128/jvi.6.1.12-19.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Funk F., Sinsheimer R. L. Process of infection with bacteriophage phiX174. 33. Templates for the synthesis of single-stranded deoxyribonucleic acid. J Virol. 1970 Mar;5(3):282–288. doi: 10.1128/jvi.5.3.282-288.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  8. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  9. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  10. Iwaya M., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins. J Mol Biol. 1971 Apr 28;57(2):159–175. doi: 10.1016/0022-2836(71)90339-1. [DOI] [PubMed] [Google Scholar]
  11. Knippers R., Razin A., Davis R., Sinsheimer R. L. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage. J Mol Biol. 1969 Oct 28;45(2):237–263. doi: 10.1016/0022-2836(69)90103-x. [DOI] [PubMed] [Google Scholar]
  12. Knippers R., Salivar W. O., Newbold J. E., Sinsheimer R. L. The process of infection with bacteriophage phiX174. XXVI. Transfer of the parental DNA of bacteriophage phiX174 into progeny bacteriophage particles. J Mol Biol. 1969 Feb 14;39(3):641–654. doi: 10.1016/0022-2836(69)90150-8. [DOI] [PubMed] [Google Scholar]
  13. Knippers R., Sinsheimer R. L. Process of infection with bacteriophage phiX174. XX. Attachment of the parental DNA of bacteriophage phiX174 to a fast-sedimenting cell component. J Mol Biol. 1968 May 28;34(1):17–29. doi: 10.1016/0022-2836(68)90231-3. [DOI] [PubMed] [Google Scholar]
  14. Komano T., Knippers R., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. XXII. Synthesis of progeny single-stranded DNA. Proc Natl Acad Sci U S A. 1968 Mar;59(3):911–916. doi: 10.1073/pnas.59.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lindqvist B. H., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J Mol Biol. 1967 Nov 28;30(1):69–80. doi: 10.1016/0022-2836(67)90244-6. [DOI] [PubMed] [Google Scholar]
  16. Razin A., Sinsheimer R. L. Replicative form II DNA of phi X174; resistance to exonucleolytic cleavage by E. coli DNA polymerase. Proc Natl Acad Sci U S A. 1970 Jul;66(3):646–650. doi: 10.1073/pnas.66.3.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Siden E. J., Hayashi M. Role of the gene beta-product in bacteriophage phi-X174 development. J Mol Biol. 1974 Oct 15;89(1):1–16. doi: 10.1016/0022-2836(74)90159-4. [DOI] [PubMed] [Google Scholar]
  18. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  19. Sinsheimer R. L., Knippers R., Komano T. Stages in the replication of bacteriophage phi X174 DNA in vivo. Cold Spring Harb Symp Quant Biol. 1968;33:443–447. doi: 10.1101/sqb.1968.033.01.051. [DOI] [PubMed] [Google Scholar]
  20. Weisbeek P. J., Sinsheimer R. L. A DNA-protein complex involved in bacteriophage phi chi 174 particle formation. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3054–3058. doi: 10.1073/pnas.71.8.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]

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