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. 1976 May;18(2):375–382. doi: 10.1128/jvi.18.2.375-382.1976

Isolation and Properties of the Replicase of Encephalomyocarditis Virus

A Traub 1, B Diskin 1, H Rosenberg 1, E Kalmar 1
PMCID: PMC515562  PMID: 5614

Abstract

The RNA-dependent RNA polymerase (replicase) of encephalomyocarditis (EMC) virus was found to be closely associated with the smooth membranes of infected BHK-21 cells. An RNA-dependent EMC replicase was extracted from the membranes with 0.15% sodium dodecyl sulfate (SDS) and 1,1,2-trichlorotri-fluoroethane (Genetron 113) and further purified by high-salt dextran-polyethylene glycol phase separation, sievorptive chromatography, and glycerol gradient sedimentation. The enzyme does not manifest strict specificity toward EMC RNA template. It can use also Qβ RNA, rRNA of BHK cells, or poly(C). SDS-polyacrylamide gel electrophoresis of purified EMC replicase labeled with radioactive methionine revealed that, of all the stable EMC proteins, the enzyme contains predominantly the 56,000-dalton (E) polypeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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