Abstract
The interaction of polyoma virus with a continuous line of rat cells was studied. Infection of these cells with polyoma did not cause virus multiplication but induced transformation. Transformed cells did not produce infectious virus, but in all clones tested virus was rescuable upon fusion with permissive mouse cells. Transformed rat cells contained, in addition to integrated viral genomes, 20 to 50 copies of nonintegrated viral DNA equivalents per cell (average). "Free" viral DNA molecules were also found in cells transformed by the ts-a and ts-8 polyoma mutants and kept at 33 C. This was not due to a virus carrier state, since the number of nonintegrated viral DNA molecules was found to be unchanged when cells were grown in the presence of antipolyoma serum. Recloning of the transformed cell lines produced subclones, which also contained free viral DNA. Most of these molecules were supercoiled and were found in the muclei of the transformed cells. The nonintegrated viral DNA is infectious. Its specifici infectivity is, however, about 100-fold lower than that of polyoma DNA extracted from productively infected cells, suggesting that these molecules contain a large proportion of defectives.
Full text
PDF








Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Basilico C., Matsuya Y., Green H. The interaction of polyoma virus with mouse-hamster somatic hybrid cells. Virology. 1970 Jun;41(2):295–305. doi: 10.1016/0042-6822(70)90082-6. [DOI] [PubMed] [Google Scholar]
- Basilico C., Wang R. Susceptibility to superinfection of hybrids between polyoma "transformed" BHK and "normal" 3T3 cells. Nat New Biol. 1971 Mar 24;230(12):105–107. doi: 10.1038/newbio230105a0. [DOI] [PubMed] [Google Scholar]
- Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Di Mayorca G., Callender J., Marin G., Giordano R. Temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):126–133. doi: 10.1016/0042-6822(69)90134-2. [DOI] [PubMed] [Google Scholar]
- FRASER K. B., GHARPURE M. Immunoofluorescent tracing of polyoma virus in transformation experiments with BHK 21 cells. Virology. 1962 Nov;18:505–507. doi: 10.1016/0042-6822(62)90048-x. [DOI] [PubMed] [Google Scholar]
- FRIED M. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Mar;53:486–491. doi: 10.1073/pnas.53.3.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fogel M. Induction of virus synthesis in polyoma-transformed cells by DNA antimetabolites and by irradiation after pretreatment with 5-bromodeoxyuridine. Virology. 1972 Jul;49(1):12–22. doi: 10.1016/s0042-6822(72)80003-5. [DOI] [PubMed] [Google Scholar]
- Fogel M., Sachs L. The activation of virus synthesis in polyoma-transformed cells. Virology. 1969 Mar;37(3):327–334. doi: 10.1016/0042-6822(69)90216-5. [DOI] [PubMed] [Google Scholar]
- Folk W. R. Induction of virus synthesis in polyoma-transformed BHK-21 cells. J Virol. 1973 Mar;11(3):424–431. doi: 10.1128/jvi.11.3.424-431.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freeman A. E., Gilden R. V., Vernon M. L., Wolford R. G., Hugunin P. E., Huebner R. J. 5-Bromo-2'-deoxyuridine potentiation of transformation of rat-embryo cells induced in vitro by 3-methylcholanthrene: induction of rat leukemia virus gs antigen in transformed cells. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2415–2419. doi: 10.1073/pnas.70.8.2415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kimura G., Itagaki A., Summers J. Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives. Int J Cancer. 1975 Apr 15;15(4):694–706. doi: 10.1002/ijc.2910150419. [DOI] [PubMed] [Google Scholar]
- Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
- Pagano J. S., McCutchan J. H., Vaheri A. Factors influencing the enhancement of the infectivity of poliovirus ribonucleic acid by diethylaminoethyl-dextran. J Virol. 1967 Oct;1(5):891–897. doi: 10.1128/jvi.1.5.891-897.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prasad I., Zouzias D., Basilico C. Simian virus 40 integration sites in the genome of virus-transformed mouse cells. J Virol. 1975 Oct;16(4):897–904. doi: 10.1128/jvi.16.4.897-904.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
- Toniolo D., Meiss H. K., Basilico C. A temperature-sensitive mutation affecting 28S ribosomal RNA production in mammalian cells. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1273–1277. doi: 10.1073/pnas.70.4.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watkins J. F., Dulbecco R. Production of SV40 virus in heterokaryons of transformed and susceptible cells. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1396–1403. doi: 10.1073/pnas.58.4.1396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watkins J. F. The SV40 rescue problem. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):355–362. doi: 10.1101/sqb.1974.039.01.046. [DOI] [PubMed] [Google Scholar]