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Journal of Virology logoLink to Journal of Virology
. 1976 May;18(2):785–787. doi: 10.1128/jvi.18.2.785-787.1976

Method for the isolation of bacteriphage T4 mutants that produce particles with giant capsids.

J V Paietta, T L McDonald, A H Doermann
PMCID: PMC515608  PMID: 1271524

Abstract

An efficient procedure is described for isolating bacteriophage T4 mutants that produce particles with giant capsids. The selective parameters of the technique rely primarily on the giants' characteristic density in cesium gradients and on their resistance to UV radiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aebi U., Bijlenga R., v d Broek J., v d Broek H., Eiserling F., Kellenberger C., Kellenberger E., Mesyanzhinov V., Müller L., Showe M. The transformation of tau particles into T4 heads. II. Transformations of the surface lattice and related observations on form determination. J Supramol Struct. 1974;2(2-4):253–275. doi: 10.1002/jss.400020218. [DOI] [PubMed] [Google Scholar]
  2. Chase M, Doermann A H. High Negative Interference over Short Segments of the Genetic Structure of Bacteriophage T4. Genetics. 1958 May;43(3):332–353. doi: 10.1093/genetics/43.3.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doermann A. H., Eiserling F. A., Boehner L. Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants. J Virol. 1973 Aug;12(2):374–385. doi: 10.1128/jvi.12.2.374-385.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doermann A. H. Lysis and Lysis Inhibition with Escherichia coli Bacteriophage. J Bacteriol. 1948 Feb;55(2):257–276. doi: 10.1128/jb.55.2.257-276.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. STREISINGER G. Phenotypic mixing of host range and serological specificities in bacteriophages T2 and T4. Virology. 1956 Jun;2(3):388–398. doi: 10.1016/0042-6822(56)90033-2. [DOI] [PubMed] [Google Scholar]

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