FIG 4 .

NDP52, p62, and LC3 colocalize with ubiquitin on C. muridarum inclusions in A549 cells. (A to F) A549 cells expressing p62-GFP (A and D) or LC3-GFP (C, E, and F) or untransfected cells (B) were infected with C. muridarum, primed with IFN-γ (100 U/ml) at 3 hpi, fixed at 20 hpi, and stained with antibodies against the indicated host proteins. Hoechst staining was used to visualize bacterial DNA of inclusions. Representative images of colocalization between indicated host markers at C. muridarum inclusions are shown. At least 50 inclusions were counted for each condition. Data are representative of three independent experiments. The values in the graphs are means ± SD (error bars). Ub −, ubiquitin negative; Ub +, ubiquitin positive.