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. 1977 Mar;21(3):1056–1060. doi: 10.1128/jvi.21.3.1056-1060.1977

Proteins of bovine leukemia virus. I. Characterization and reactions with natural antibodies.

L Deshayes, D Levy, A L Parodi, J P Levy
PMCID: PMC515645  PMID: 191635

Abstract

The bovine leukemia virus (BLV) was purified from a chronically infected fetal lamb kidney cell line. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of this virus revealed the presence of eight distinguishable viral components with molecular weights ranging from 80,000 to 11,000. The major component is a non-glycosylated protein having a molecular weight of 24,000 (p24). At least three heavier polypeptides were found, one of them representing a glycoprotein (gp 60). In addition, four minor polypeptides with respective molecular weights of 19,000, 16,000, 13,000, and 11,000 were identified. In a complement fixation assay using naturally occurring antibodies of a leukemic cow, four polypeptides, which included gp 60, p35, p24, and p16, were found to be reactive.

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Selected References

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