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. 1977 Mar;21(3):1196–1204. doi: 10.1128/jvi.21.3.1196-1204.1977

Identification of the defective genes in three mutant groups of influenza virus.

M B Ritchey, P Palese
PMCID: PMC515660  PMID: 850307

Abstract

Seven complementation-recombination groups of temperature-sensitive (ts) influenza WSN virus mutants have been previously isolated. Recently two of these groups (IV and VI) were shown to possess defects in the neuraminidase and the hemagglutinin gene, respectively, and two groups (I and III) were reported to have defects in the P3 and P1 proteins which are required for complementary RNA synthesis. In this communication we report on the defects in the remaining three mutant groups. Wild-type (ts+) recombinants derived from ts mutants and different non-ts influenza viruses were analyzed on RNA polyacrylamide gels. This technique permitted the identification of the P2 protein, the nucleoprotein, and the M protein as the defective gene products in mutant groups II, V, and VII, respectively. Based on the physiological behavior of mutants in groups II and V, it appears that P2 protein and nucleoprotein are required for virion RNA synthesis during influenza virus replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bean W. J., Jr, Simpson R. W. Transcriptase activity and genome composition of defective influenza virus. J Virol. 1976 Apr;18(1):365–369. doi: 10.1128/jvi.18.1.365-369.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Floyd R. W., Stone M. P., Joklik W. K. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Anal Biochem. 1974 Jun;59(2):599–609. doi: 10.1016/0003-2697(74)90313-3. [DOI] [PubMed] [Google Scholar]
  3. Ghendon Y. Z., Markushin S. G., Marchenko A. T., Sitnikov B. S., Ginzburg V. P. Biochemical characteristics of fowl plague virus TS mutants. Virology. 1973 Oct;55(2):305–319. doi: 10.1016/0042-6822(73)90170-0. [DOI] [PubMed] [Google Scholar]
  4. Hirst G. K. Mechanism of influenza recombination. I. Factors influencing recombination rates between temperature-sensitive mutants of strain WSN and the classification of mutants into complementation--recombination groups. Virology. 1973 Sep;55(1):81–93. doi: 10.1016/s0042-6822(73)81010-4. [DOI] [PubMed] [Google Scholar]
  5. Klenk H. D., Rott R. Formation of influenza virus proteins. J Virol. 1973 Jun;11(6):823–831. doi: 10.1128/jvi.11.6.823-831.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Krug R. M. Influenza viral RNPs newly synthesized during the latent period of viral growth in MDCK cells. Virology. 1971 Apr;44(1):125–136. doi: 10.1016/0042-6822(71)90159-0. [DOI] [PubMed] [Google Scholar]
  7. Krug R. M., Ueda M., Palese P. Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis. J Virol. 1975 Oct;16(4):790–796. doi: 10.1128/jvi.16.4.790-796.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lazarowitz S. G., Compans R. W., Choppin P. W. Influenza virus structural and nonstructural proteins in infected cells and their plasma membranes. Virology. 1971 Dec;46(3):830–843. doi: 10.1016/0042-6822(71)90084-5. [DOI] [PubMed] [Google Scholar]
  9. Mackenzie J. S., Dimmock N. J. A preliminary study of physiological characteristics of temperature-sensitive mutants of influenza virus. J Gen Virol. 1973 Apr;19(1):51–63. doi: 10.1099/0022-1317-19-1-51. [DOI] [PubMed] [Google Scholar]
  10. Mackenzie J. S. Isolation of temperature-sensitive mutants and the construction of a preliminary genetic map for influenza virus. J Gen Virol. 1970 Jan;6(1):63–75. doi: 10.1099/0022-1317-6-1-63. [DOI] [PubMed] [Google Scholar]
  11. Palese P., Ritchey M. B., Schulman J. L., Kilbourne E. D. Genetic composition of a high-yielding influenza A virus recombinant: a vaccine strain against "Swine" influenza. Science. 1976 Oct 15;194(4262):334–335. doi: 10.1126/science.968486. [DOI] [PubMed] [Google Scholar]
  12. Palese P., Ritchey M. B., Schulman J. L. Mapping of the influenza virus genome. II. Identification of the P1, P2, and P3 genes. Virology. 1977 Jan;76(1):114–121. doi: 10.1016/0042-6822(77)90288-4. [DOI] [PubMed] [Google Scholar]
  13. Palese P., Ritchey M. B., Schulman J. L. P1 and P3 proteins of influenza virus are required for complementary RNA synthesis. J Virol. 1977 Mar;21(3):1187–1195. doi: 10.1128/jvi.21.3.1187-1195.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Palese P., Schulman J. L. Differences in RNA patterns of influenza A viruses. J Virol. 1976 Mar;17(3):876–884. doi: 10.1128/jvi.17.3.876-884.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Palese P., Schulman J. L. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142–2146. doi: 10.1073/pnas.73.6.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Palese P., Schulman J. L. RNA pattern of 'swine' influenza virus isolated from man is similar to those of other swine influenza viruses. Nature. 1976 Oct 7;263(5577):528–530. doi: 10.1038/263528a0. [DOI] [PubMed] [Google Scholar]
  17. Palese P. The genes of influenza virus. Cell. 1977 Jan;10(1):1–10. doi: 10.1016/0092-8674(77)90133-7. [DOI] [PubMed] [Google Scholar]
  18. Palese P., Tobita K., Ueda M., Compans R. W. Characterization of temperature sensitive influenza virus mutants defective in neuraminidase. Virology. 1974 Oct;61(2):397–410. doi: 10.1016/0042-6822(74)90276-1. [DOI] [PubMed] [Google Scholar]
  19. Pons M. W. A reexamination of influenza single-and double-stranded RNAs by gel electrophoresis. Virology. 1976 Feb;69(2):789–792. doi: 10.1016/0042-6822(76)90508-0. [DOI] [PubMed] [Google Scholar]
  20. Richey M. B., Palese P. In vitro translation of influenza virus messenger RNAs. Virology. 1976 Jul 15;72(2):410–420. doi: 10.1016/0042-6822(76)90170-7. [DOI] [PubMed] [Google Scholar]
  21. Ritchey M. B., Palese P., Kilbourne E. D. RNAs of influenza A, B, and C viruses. J Virol. 1976 May;18(2):738–744. doi: 10.1128/jvi.18.2.738-744.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ritchey M. B., Palese P., Schulman J. L. Difference in protein patterns of influenza A viruses. Virology. 1977 Jan;76(1):122–128. doi: 10.1016/0042-6822(77)90289-6. [DOI] [PubMed] [Google Scholar]
  23. Ritchey M. B., Palese P., Schulman J. L. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein. J Virol. 1976 Oct;20(1):307–313. doi: 10.1128/jvi.20.1.307-313.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Scholtissek C., Bowles A. L. Isolation and characterization of temperature-sensitive mutants of fowl plague virus. Virology. 1975 Oct;67(2):576–587. doi: 10.1016/0042-6822(75)90457-2. [DOI] [PubMed] [Google Scholar]
  25. Scholtissek C., Harms E., Rohde W., Orlich M., Rott R. Correlation between RNA fragments of fowl plague virus and their corresponding gene functions. Virology. 1976 Oct 15;74(2):332–344. doi: 10.1016/0042-6822(76)90340-8. [DOI] [PubMed] [Google Scholar]
  26. Scholtissek C., Kruczinna R., Rott R., Klenk H. D. Characteristics of an influenza mutant temperature-sensitive for viral RNA synthesis. Virology. 1974 Apr;58(2):317–322. doi: 10.1016/0042-6822(74)90067-1. [DOI] [PubMed] [Google Scholar]
  27. Simpson R. W., Hirst G. K. Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology. 1968 May;35(1):41–49. doi: 10.1016/0042-6822(68)90303-6. [DOI] [PubMed] [Google Scholar]
  28. Spring S. B., Nusinoff S. R., Tierney E. L., Richman D. D., Murphy B. R., Chanock R. M. Temperature-sensitive mutants of influenza. VIII. Genetic and biological characterization of TS mutants of influenza virus A (H3N2) and their assignment to complementation groups. Virology. 1975 Aug;66(2):542–550. doi: 10.1016/0042-6822(75)90226-3. [DOI] [PubMed] [Google Scholar]
  29. Sugiura A., Tobita K., Kilbourne E. D. Isolation and preliminary characterization of temperature-sensitive mutants of influenza virus. J Virol. 1972 Oct;10(4):639–647. doi: 10.1128/jvi.10.4.639-647.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sugiura A., Ueda M., Tobita K., Enomoto C. Further isolation and characterization of temperature-sensitive mutants of influenza virus. Virology. 1975 Jun;65(2):363–373. doi: 10.1016/0042-6822(75)90042-2. [DOI] [PubMed] [Google Scholar]
  31. Ueda M., Kilbourne E. D. Temperature-sensitive mutants of influenza virus: a mutation in the hemagglutinin gene. Virology. 1976 Apr;70(2):425–431. doi: 10.1016/0042-6822(76)90283-x. [DOI] [PubMed] [Google Scholar]
  32. Ueda M. Temperature-sensitive mutants of influenza virus. Isolation and preliminary characterization. Arch Gesamte Virusforsch. 1972;39(4):360–368. doi: 10.1007/BF01241015. [DOI] [PubMed] [Google Scholar]

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