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. 1977 Mar;21(3):1240–1242. doi: 10.1128/jvi.21.3.1240-1242.1977

Shutoff of Host RNA Synthesis in Bacteriophage T4-Infected Escherichia coli in the Absence of Host DNA Degradation and Nuclear Disruption

D Peter Snustad 1, Claudia J H Bursch 1
PMCID: PMC515669  PMID: 321802

Abstract

In contrast to its effect on host DNA synthesis, nuclear disruption in phage T4-infected Escherichia coli B/5 cells has no effect on the shutoff of host RNA synthesis. Host RNA synthesis is shut off normally after infection with T4 multiple mutants that fail to induce both nuclear disruption and host DNA degradation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bautz F. A., Bautz E. K. Mapping of deletions in a non-essential region of the phage T4 genome. J Mol Biol. 1967 Sep 14;28(2):345–355. doi: 10.1016/s0022-2836(67)80014-7. [DOI] [PubMed] [Google Scholar]
  3. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  4. Depew R. E., Snopek T. J., Cozzarelli N. R. Characterization of a new class of deletions of the D region of the bacteriophage T4 genome. Virology. 1975 Mar;64(1):144–145. doi: 10.1016/0042-6822(75)90086-0. [DOI] [PubMed] [Google Scholar]
  5. Duckworth D. H. Biological activity of bacteriophage ghosts and "take-over" of host functions by bacteriophage. Bacteriol Rev. 1970 Sep;34(3):344–363. doi: 10.1128/br.34.3.344-363.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
  7. Kozinski A. W., Lin T. H. Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA. Proc Natl Acad Sci U S A. 1965 Jul;54(1):273–278. doi: 10.1073/pnas.54.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parson K. A., Snustad D. P. Host DNA degradation after infection of Escherichia coli with bacteriophage T4: dependence of the alternate pathway of degradation which occurs in the absence of both T4 endonuclease II and nuclear disruption on T4 endonuclease IV. J Virol. 1975 Jan;15(1):221–224. doi: 10.1128/jvi.15.1.221-224.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sadowski P. D., Vetter D. Control of T4 endonuclease IV by the D2a region of bacteriophage T4. Virology. 1973 Aug;54(2):544–546. doi: 10.1016/0042-6822(73)90165-7. [DOI] [PubMed] [Google Scholar]
  10. Sadowski P. D., Warner H. R., Hercules K., Munro J. L., Mendelsohn S., Wiberg J. S. Mutants of bacteriophage T4 defective in the induction of T4 endonuclease II. J Biol Chem. 1971 May 25;246(10):3431–3433. [PubMed] [Google Scholar]
  11. Snustad D. P., Bursch C. J., Parson K. A., Hefeneider S. H. Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption: shutoff of host DNA and protein synthesis gene dosage experiments, identification of a restrictive host, and possible biological significance. J Virol. 1976 Apr;18(1):268–288. doi: 10.1128/jvi.18.1.268-288.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Snustad D. P., Conroy L. M. Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption. I. Isolation and genetic characterization. J Mol Biol. 1974 Nov 15;89(4):663–673. doi: 10.1016/0022-2836(74)90043-6. [DOI] [PubMed] [Google Scholar]
  13. Snustad D. P., Parson K. A., Warner H. R., Tutas D. J., Wehner J. M., Koerner J. F. Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption. II. Physiological state of the host nucleoid in infected cells. J Mol Biol. 1974 Nov 15;89(4):675–687. doi: 10.1016/0022-2836(74)90044-8. [DOI] [PubMed] [Google Scholar]
  14. Warner H. R., Hobbs M. D. Incorporation of uracil-14C into nucleic acids in Escherichia coli infected with bacteriophage T4 and T4 amber mutants. Virology. 1967 Nov;33(3):376–384. doi: 10.1016/0042-6822(67)90113-4. [DOI] [PubMed] [Google Scholar]
  15. Warner H. R., Snustad P., Jorgensen S. E., Koerner J. F. Isolation of bacteriophage T4 mutants defective in the ability to degrade host deoxyribonucleic acid. J Virol. 1970 Jun;5(6):700–708. doi: 10.1128/jvi.5.6.700-708.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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