Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Apr;22(1):113–117. doi: 10.1128/jvi.22.1.113-117.1977

Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.

B Norrild, B F Vestergaard
PMCID: PMC515691  PMID: 192909

Abstract

Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.

Full text

PDF
113

Images in this article

114Image
on p.114
115Image
on p.115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bjerrum O. J., Bog-Hansen T. C. The immunochemical approach to the characterization of membrane proteins. Human erythrocyte membrane proteins analysed as a model system. Biochim Biophys Acta. 1976 Nov 11;455(1):66–89. doi: 10.1016/0005-2736(76)90154-1. [DOI] [PubMed] [Google Scholar]
  2. Bone D. R., Courtney R. J. A temperature-sensitive mutant of herpes simplex virus type 1 defective in the synthesis of the major capsid polypeptide. J Gen Virol. 1974 Jul;24(1):17–27. doi: 10.1099/0022-1317-24-1-17. [DOI] [PubMed] [Google Scholar]
  3. Courtney R. J., Benyesh-Melnick M. Isolation and characterization of a large molecular-weight polypeptide of herpes simplex virus type 1. Virology. 1974 Dec;62(2):539–551. doi: 10.1016/0042-6822(74)90414-0. [DOI] [PubMed] [Google Scholar]
  4. Courtney R. J., McCombs R. M., Benyesh-Melnick M. Antigens specified by herpesviruses. II. Effect of arginine deprivation on the synthesis of cytoplasmic and nuclear proteins. Virology. 1971 Feb;43(2):356–365. doi: 10.1016/0042-6822(71)90308-4. [DOI] [PubMed] [Google Scholar]
  5. Courtney R. J., Powell K. L. Immunological and biochemical characterization of polypeptides induced by herpes simplex virus types 1 and 2. IARC Sci Publ. 1975;(11 Pt 1):63–73. [PubMed] [Google Scholar]
  6. Heine J. W., Honess R. W., Cassai E., Roizman B. Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. J Virol. 1974 Sep;14(3):640–651. doi: 10.1128/jvi.14.3.640-651.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heine J. W., Spear P. G., Roizman B. Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. J Virol. 1972 Mar;9(3):431–439. doi: 10.1128/jvi.9.3.431-439.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Honess R. W., Roizman B. Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides. J Virol. 1975 Nov;16(5):1308–1326. doi: 10.1128/jvi.16.5.1308-1326.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honess R. W., Watson D. H. Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. J Gen Virol. 1974 Feb;22(2):171–185. doi: 10.1099/0022-1317-22-2-171. [DOI] [PubMed] [Google Scholar]
  10. McCombs R. M. Antigens specified by herpesviruses. 3. Viral-induced nuclear polypeptides. Virology. 1974 Feb;57(2):448–458. doi: 10.1016/0042-6822(74)90184-6. [DOI] [PubMed] [Google Scholar]
  11. Powell K. L., Buchan A., Sim C., Watson D. H. Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody. Nature. 1974 May 24;249(455):360–361. doi: 10.1038/249360a0. [DOI] [PubMed] [Google Scholar]
  12. Powell K. L., Watson D. H. Some structural antigens of herpes simplex virus type 1. J Gen Virol. 1975 Nov;29(2):167–178. doi: 10.1099/0022-1317-29-2-167. [DOI] [PubMed] [Google Scholar]
  13. Roizman B., Hayward G., Jacob R., Wadsworth S., Frenkel N., Honess R. W., Kozak M. Human herpersviruses I: a model for molecular organization and regulation of herpesviruses-a review. IARC Sci Publ. 1975;(11 Pt 1):3–38. [PubMed] [Google Scholar]
  14. Spear P. G., Roizman B. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J Virol. 1972 Jan;9(1):143–159. doi: 10.1128/jvi.9.1.143-159.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steck T. L. The organization of proteins in the human red blood cell membrane. A review. J Cell Biol. 1974 Jul;62(1):1–19. doi: 10.1083/jcb.62.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vestergaard B. F. Crossed immunoelectrophoretic characterization of Herpesvirus hominis type 1 and 2 antigens. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):808–810. doi: 10.1111/j.1699-0463.1973.tb02282.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES