Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Apr;22(1):168–174. doi: 10.1128/jvi.22.1.168-174.1977

Isolation and comparison of murine leukemia virus-related glycoproteins from AKR and New Zealand mice.

S J Kennel
PMCID: PMC515697  PMID: 192911

Abstract

The major glycoprotein (gp70) of murine leukemia virus occurs free of virus in the serum and body fluids of certain strains of mice. These glycoproteins were isolated from New Zealand Black mouse (NZB) ascites fluid and from AKR and New Zealand White mouse (NZW) serum by immunoaffinity chromatography and were compared by immunological tests and peptide mapping. Glycoproteins gp70-NZB and gp70-NZW were indistinguishable by all criteria tested and were more closely related to gp70 from Moloney leukemia virus than was gp70-AKR.

Full text

PDF
168

Images in this article

170Image
on p.170
171Image
on p.171

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beatty P. G., Kim B. S., Rowley D. A., Coppleson L. W. Antibody against the antigen receptor of a plasmacytoma prolongs survival of mice bearing the tumor. J Immunol. 1976 May;116(5):1391–1396. [PubMed] [Google Scholar]
  2. Del Villano B. C., Lerner R. A. Relationship between the oncornavirus gene product gp70 and a major protein secretion of the mouse genital tract. Nature. 1976 Feb 12;259(5543):497–499. doi: 10.1038/259497a0. [DOI] [PubMed] [Google Scholar]
  3. Gibson W. Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology. 1974 Dec;62(2):319–336. doi: 10.1016/0042-6822(74)90395-x. [DOI] [PubMed] [Google Scholar]
  4. Hino S., Stephenson J. R., Aaronson S. A. Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. J Virol. 1976 Jun;18(3):933–941. doi: 10.1128/jvi.18.3.933-941.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hunsmann G., Claviez M., Moennig V., Schwarz H., Schäfer W. Properties of mouse leukemia viruses. X. Occurrence of viral structural antigens on the cell surface as revealed by a cytotoxicity test. Virology. 1976 Jan;69(1):157–168. doi: 10.1016/0042-6822(76)90203-8. [DOI] [PubMed] [Google Scholar]
  6. Hunsmann G., Moennig V., Pister L., Seifert E., Schäfer W. Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology. 1974 Dec;62(2):307–318. doi: 10.1016/0042-6822(74)90394-8. [DOI] [PubMed] [Google Scholar]
  7. Ihle J. N., Domotor J. J., Jr, Bengali K. M. Characterization of the type and group specificities of the immune response in mice to murine leukemia viruses. J Virol. 1976 Apr;18(1):124–131. doi: 10.1128/jvi.18.1.124-131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ihle J. N., Yurconic M., Jr, Hanna M. G., Jr Autogenous immunity to endogenous RNA tumor virus. Radioimmune precipitation assay of mouse serum antibody levels. J Exp Med. 1973 Jul 1;138(1):194–208. doi: 10.1084/jem.138.1.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kennel S. J., Del Villano B. C., Levy R. L., Lerner R. A. Properties of an oncornavirus glycoprotein: evidence for its presence on the surface of virions and infected cells. Virology. 1973 Oct;55(2):464–475. doi: 10.1016/0042-6822(73)90188-8. [DOI] [PubMed] [Google Scholar]
  10. Kennel S. J., Feldman J. D. Distribution of viral glycoprotein gp 69/71 on cell surfaces of producer and nonproducer cells. Cancer Res. 1976 Jan;36(1):200–208. [PubMed] [Google Scholar]
  11. Kennel S. J. Purification of a glycoprotein from mouse ascites fluid by immunoaffinity chromatography which is related to the major glycoprotein of murine leukemia viruses. Immunologic and structural comparison with purified viral glycoproteins. J Biol Chem. 1976 Oct 25;251(20):6197–6204. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lerner R. A., Wilson C. B., Villano B. C., McConahey P. J., Dixon F. J. Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycorprotein, gp70. J Exp Med. 1976 Jan 1;143(1):151–166. doi: 10.1084/jem.143.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  16. Morrison T. G. Site of synthesis of membrane and nonmembrane proteins of vesicular stomatitis virus. J Biol Chem. 1975 Sep 10;250(17):6955–6962. [PubMed] [Google Scholar]
  17. Schwarz H., Hunsmann G., Moenning V., Schäfer W. Properties of mouse leukemia viruses. XI. Immunoelectron microscopic studies on viral structural antigens on the cell surface. Virology. 1976 Jan;69(1):169–178. doi: 10.1016/0042-6822(76)90204-x. [DOI] [PubMed] [Google Scholar]
  18. Stephension J. R., Reynolds R. K., Tronick S. R., Aaronson S. A. Distribution of three classes of endogenous type-C RNA viruses among inbred strains of mice. Virology. 1975 Oct;67(2):404–414. doi: 10.1016/0042-6822(75)90442-0. [DOI] [PubMed] [Google Scholar]
  19. Stephenson J. R., Tronick S. R., Aaronson S. A. Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-Type viruses. Virology. 1974 Mar;58(1):1–8. doi: 10.1016/0042-6822(74)90135-4. [DOI] [PubMed] [Google Scholar]
  20. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strand M., August J. T. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen. J Biol Chem. 1973 Aug 25;248(16):5627–5633. [PubMed] [Google Scholar]
  22. Strand M., Lilly F., August J. T. Genetic control of the expression of murine leukemia virus proteins in tissues of normal, young adult mice. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1117–1122. doi: 10.1101/sqb.1974.039.01.127. [DOI] [PubMed] [Google Scholar]
  23. Teramoto Y. A., Puentes M. J., Young L. J., Cardiff R. D. Structure of the mouse mammary tumor virus: polypeptides and glycoproteins. J Virol. 1974 Feb;13(2):411–418. doi: 10.1128/jvi.13.2.411-418.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tung J. S., Shen F. W., Fleissner E., Boyse E. A. X-gp70: a third molecular species of the envelope protein gp70 of murine leukemia virus, expressed on mouse lymphoid cells. J Exp Med. 1976 Apr 1;143(4):969–974. doi: 10.1084/jem.143.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Witte O. N., Weissman I. L. Membrane proteins of MSV-MLV: their role in virion-virion interactions in vitro. Virology. 1974 Oct;61(2):588–593. doi: 10.1016/0042-6822(74)90292-x. [DOI] [PubMed] [Google Scholar]
  26. Witte O. N., Weissman I. L. Polypeptides of Moloney sarcoma-leukemia virions: their resolution and incorporation into extracellular virions. Virology. 1974 Oct;61(2):575–587. doi: 10.1016/0042-6822(74)90291-8. [DOI] [PubMed] [Google Scholar]
  27. Yoshiki T., Mellors R. C., Strand M., August J. T. The viral envelope glycoprotein of murine leukemia virus and the pathogenesis of immune complex glomerulonephritis of New Zealand mice. J Exp Med. 1974 Oct 1;140(4):1011–1027. doi: 10.1084/jem.140.4.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES