Abstract
Hepatitis A virus antigen was purified from early acute-phase chimpanzee stools by a rapid three-step procedure using 7% polyethylene glycol precipitation, CsCl banding, and Sepharose 2B column chromatography. Electron microscopic examination of the hepatitis A virus entigen preparation revealed highly purified hepatitis A virus particles.
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- Bradley D. W., Hollinger F. B., Hornbeck C. L., Maynard J. E. Isolation and characterization of hepatitis A virus. Am J Clin Pathol. 1976 May;65(5 Suppl):876–889. [PubMed] [Google Scholar]
- Bradley D. W., Hornbeck C. L., Cood E. H., Maynard J. E., Gravelle C. R. CsCl banding of hepatitis A-associated virus-like particles. J Infect Dis. 1975 Mar;131(3):304–306. doi: 10.1093/infdis/131.3.304. [DOI] [PubMed] [Google Scholar]
- Deinhardt F., Peterson D., Cross G., Wolfe L., Holmes A. W. Hepatitis in marmosets. Am J Med Sci. 1975 Jul-Aug;270(1):73–80. doi: 10.1097/00000441-197507000-00011. [DOI] [PubMed] [Google Scholar]
- Dienstag J. L., Feinstone S. M., Purcell R. H., Hoofnagle J. H., Barker L. F., London W. T., Popper H., Peterson J. M., Kapikian A. Z. Experimental infection of chimpanzees with hepatitis A virus. J Infect Dis. 1975 Nov;132(5):532–545. doi: 10.1093/infdis/132.5.532. [DOI] [PubMed] [Google Scholar]
- Feinstone S. M., Kapikian A. Z., Gerin J. L., Purcell R. H. Buoyant density of the hepatitis A virus-like particle in cesium chloride. J Virol. 1974 Jun;13(6):1412–1414. doi: 10.1128/jvi.13.6.1412-1414.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
- Gravelle C. R., Hornbeck C. L., Maynard J. E., Schable C. A., Cook E. H., Bradley D. W. Hepatitis A: report of a common-source outbreak with recovery of a possible etiologic agent. II. Laboratory studies. J Infect Dis. 1975 Feb;131(2):167–171. doi: 10.1093/infdis/131.2.167. [DOI] [PubMed] [Google Scholar]
- Hollinger F. B., Bradley D. W., Maynard J. E., Dreesman G. R., Melnick J. L. Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975 Nov;115(5):1464–1466. [PubMed] [Google Scholar]
- Maynard J. E., Bradley D. W., Gravelle C. R., Ebert J. W., Krushak D. H. Preliminary studies of hepatitis A in chimpanzees. J Infect Dis. 1975 Feb;131(2):194–197. doi: 10.1093/infdis/131.2.194. [DOI] [PubMed] [Google Scholar]
- Maynard J. E., Lorenz D., Bradley D. W., Feinstone S. M., Krushak D. H., Barker L. F., Purcell R. H. Review of infectivity studies in nonhuman primates with virus-like particles associated with MS-1 hepatitis. Am J Med Sci. 1975 Jul-Aug;270(1):81–85. doi: 10.1097/00000441-197507000-00012. [DOI] [PubMed] [Google Scholar]
- Provost P. J., Wolanski B. S., Miller W. J., Ittensohn O. L., McAleer W. J., Hilleman M. R. Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578). Proc Soc Exp Biol Med. 1975 Feb;148(2):532–539. doi: 10.3181/00379727-148-38578. [DOI] [PubMed] [Google Scholar]
- Purcell R. H., Wong D. C., Moritsugu Y., Dienstag J. L., Routenberg J. A., Boggs J. D. A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody. J Immunol. 1976 Feb;116(2):349–356. [PubMed] [Google Scholar]