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. 1977 May;22(2):267–272. doi: 10.1128/jvi.22.2.267-272.1977

Cell cycle dependency of murine cytomegalovirus replication in synchronized 3T3 cells.

M T Muller, J B Hudson
PMCID: PMC515715  PMID: 194054

Abstract

Synchronized murine 3T3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. The normal latent period of 12 h characteristic of asynchronous 3T3 cells was protracted to more than 24 h after an early G1 infection in synchronous cells. In this case viral progeny were not detected until after the initiation of the host S-phase. Cells maintained in the G1 phase did not replicate virus. This failure could not be explained by a decrease in virus penetration but was apparently due to a requirement for an event associated with the host S-phase. Thymidine-induced inhibition of cell cycle traverse also blocked virus replication. Viral DNA synthesis did not initiate until after the initiation of host DNA. In contrast, herpes simplex virus type 1 replicated in 3T3 cells independently of the cell cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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