Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 May;22(2):300–307. doi: 10.1128/jvi.22.2.300-307.1977

Assay of noninfectious fragments of DNA of avian leukosis virus-infected cells by marker rescue.

G M Cooper, S B Castellot
PMCID: PMC515719  PMID: 68125

Abstract

A marker rescue assay of noninfectious fragments of avian leukosis virus DNAs is describe. DNA fragments were prepared either by sonication of EcoRI-digestion of DNAs of chicken cells infected with wild-type Rous sarcoma virus, with a nontransforming avian leukosis virus, and with a mutant of Rous sarcoma virus temperature sensitive for transformation. Recipient cultures of chicken embryo fibroblasts were treated with noninfectious DNA fragments and infected with temperature-sensitive mutants of Rous sarcoma virus defective in DNA polymerase or in an internal virion structural protein. Wild-type progeny viruses which replicated at the nonpermissive temperature were isolated. Some of the wild-type progeny acquired both the wild-type DNA polymerase and the subgroup specificity of the Rous sarcona virus strain used for preparation of sonicated or EcoRI-digested DNA fragments. Therefore the genetic markers for DNA polymerase and envelope were linked and appeared to be located on the same EcoRi fragment of the DNA of Rous sarcoma virus-infected cells.

Full text

PDF
300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battula N., Temin H. M. Infectious DNA of spleen necrosis virus is integrated at a single site in the DNA of chronically infected chicken fibroblasts. Proc Natl Acad Sci U S A. 1977 Jan;74(1):281–285. doi: 10.1073/pnas.74.1.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coffin J. M., Billeter M. A. A physical map of the Rous sarcoma virus genome. J Mol Biol. 1976 Jan 25;100(3):293–318. doi: 10.1016/s0022-2836(76)80065-4. [DOI] [PubMed] [Google Scholar]
  3. Cooper G. M., Temin H. M. Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. J Virol. 1974 Nov;14(5):1132–1141. doi: 10.1128/jvi.14.5.1132-1141.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper G. M., Temin H. M. Lack of infectivity of the endogenous avian leukosis virus-related genes in the DNA of uninfected chicken cells. J Virol. 1976 Feb;17(2):422–430. doi: 10.1128/jvi.17.2.422-430.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crittenden L. B., Smith E. J., Weiss R. A., Sarma P. S. Host gene control of endogenous avian leukosis virus production. Virology. 1974 Jan;57(1):128–138. doi: 10.1016/0042-6822(74)90114-7. [DOI] [PubMed] [Google Scholar]
  6. Fritsch E., Temin H. M. Formation and structure of infectious DNA of spleen necrosis virus. J Virol. 1977 Jan;21(1):119–130. doi: 10.1128/jvi.21.1.119-130.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  8. Graham F. L., van der Eb A. J., Heijneker H. L. Size and location of the transforming region in human adenovirus type 5 DNA. Nature. 1974 Oct 25;251(5477):687–691. doi: 10.1038/251687a0. [DOI] [PubMed] [Google Scholar]
  9. Guntaka R. V., Richards O. C., Shank P. R., Kung H. J., Davidson N. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis. J Mol Biol. 1976 Sep 15;106(2):337–357. doi: 10.1016/0022-2836(76)90090-5. [DOI] [PubMed] [Google Scholar]
  10. Haase A. T., Traynor B. L., Ventura P. E., Alling D. W. Infectivity of visna virus DNA. Virology. 1976 Mar;70(1):65–79. doi: 10.1016/0042-6822(76)90236-1. [DOI] [PubMed] [Google Scholar]
  11. Hill M., Hillova J. Virus recovery in chicken cells tested with Rous sarcoma cell DNA. Nat New Biol. 1972 May 10;237(71):35–39. doi: 10.1038/newbio237035a0. [DOI] [PubMed] [Google Scholar]
  12. Hunter E., Hayman M. J., Rongey R. W., Vogt P. K. An avian sarcoma virus mutant that is temperature sensitive for virion assembly. Virology. 1976 Jan;69(1):35–49. doi: 10.1016/0042-6822(76)90192-6. [DOI] [PubMed] [Google Scholar]
  13. Hunter E., Vogt P. K. Temperature-sensitive mutants of avian sarcoma viruses. Genetic recombination with wild type sarcoma virus and physiological analysis of multiple mutants. Virology. 1976 Jan;69(1):23–34. doi: 10.1016/0042-6822(76)90191-4. [DOI] [PubMed] [Google Scholar]
  14. Joho R. H., Billeter M. A., Weissmann C. Mapping of biological functions on RNA of avian tumor viruses: location of regions required for transformation and determination of host range. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4772–4776. doi: 10.1073/pnas.72.12.4772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lai C. J., Nathans D. Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J Mol Biol. 1974 Oct 15;89(1):179–193. doi: 10.1016/0022-2836(74)90169-7. [DOI] [PubMed] [Google Scholar]
  16. Lai C. J., Nathans D. Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology. 1974 Aug;60(2):466–475. doi: 10.1016/0042-6822(74)90340-7. [DOI] [PubMed] [Google Scholar]
  17. Linial M., Mason W. S. Characterization of two conditional early mutants of Rous sarcoma virus. Virology. 1973 May;53(1):258–273. doi: 10.1016/0042-6822(73)90484-4. [DOI] [PubMed] [Google Scholar]
  18. Mason W. S., Friis R. R., Linial M., Vogt P. K. Determination of the defective function in two mutants of Rous sarcoma virus. Virology. 1974 Oct;61(2):559–574. doi: 10.1016/0042-6822(74)90290-6. [DOI] [PubMed] [Google Scholar]
  19. Miller L. K., Fried M. Construction of the genetic map of the polyoma genome. J Virol. 1976 Jun;18(3):824–832. doi: 10.1128/jvi.18.3.824-832.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nicolson M. O., Hariri F., Krempin H. M., McAllister R. M., Gilden R. V. Infectious proviral DNA in human cells infected with transformation-defective type C viruses. Virology. 1976 Apr;70(2):301–312. doi: 10.1016/0042-6822(76)90273-7. [DOI] [PubMed] [Google Scholar]
  21. Scolnick E. M., Bumgarner S. J. Isolation of infectious xenotropic mouse type C virus by transfection of a heterologous cell with DNA from a transformed mouse cell. J Virol. 1975 May;15(5):1293–1296. doi: 10.1128/jvi.15.5.1293-1296.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smotkin D., Gianni A. M., Rozenblatt S., Weinberg R. A. Infectious viral DNA of murine leukemia virus. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4910–4913. doi: 10.1073/pnas.72.12.4910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang L. H., Duesberg P. H., Kawai S., Hanafusa H. Location of envelope-specific and sarcoma-specific oligonucleotides on RNA of Schmidt-Ruppin Rous sarcoma virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):447–451. doi: 10.1073/pnas.73.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wang L. H., Duesberg P., Beemon K., Vogt P. K. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end. J Virol. 1975 Oct;16(4):1051–1070. doi: 10.1128/jvi.16.4.1051-1070.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wang L. H., Duesberg P., Mellon P., Vogt P. K. Distribution of envelope-specific and sarcoma-specific nucleotide sequences from different parents in the RNAs of avian tumor virus recombinants. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1073–1077. doi: 10.1073/pnas.73.4.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wang L., Galehouse D., Mellon P., Duesberg P., Mason W. S., Vogt P. K. Mapping oligonucleotides of Rous sarcoma virus RNA that segregate with polymerase and group-specific antigen markers in recombinants. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3952–3956. doi: 10.1073/pnas.73.11.3952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wilkie N. M., Clements J. B., Macnab J. C., Subak-Sharpe J. H. The structure and biological properties of herpes simplex virus DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):657–666. doi: 10.1101/sqb.1974.039.01.079. [DOI] [PubMed] [Google Scholar]
  28. Wyke J. A., Linial M. Temperature-sensitive avian sarcoma viruses: a physiological comparison of twenty mutants. Virology. 1973 May;53(1):152–161. doi: 10.1016/0042-6822(73)90474-1. [DOI] [PubMed] [Google Scholar]
  29. Wyke J. A. The selective isolation of temperature-sensitive mutants of Rous sarcoma virus. Virology. 1973 Apr;52(2):587–590. doi: 10.1016/0042-6822(73)90357-7. [DOI] [PubMed] [Google Scholar]
  30. va de POL J. H., VELDHUISEN G., COHEN J. A. Phage transformation: a new criterium for the biological activity of bacteriophage DNA. Biochim Biophys Acta. 1961 Apr 1;48:417–418. doi: 10.1016/0006-3002(61)90501-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES