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. 1977 Jun;22(3):694–701. doi: 10.1128/jvi.22.3.694-701.1977

Location of the ss--mutation of bacteriophage T7 in genes 10, the structural gene for the major capsid protein.

D Vetter, L Roberts, J B Jackowski, P D Sadowski
PMCID: PMC515768  PMID: 327090

Abstract

T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by complementation studies and genetic crosses that the ss--mutation maps in gene 10, the structural gene for the major protein of the capsid. This finding implies that the gene 10 protein may interact with a host protein during phage development and that the abortive infection of T7 observed in S. sonnei D2 371-48 with T7+ phage may be a defect in head morphogenesis. Our studies also reveal that various T7 strains commonly contain deletions in nonessential regions. T7 ss--mutants selected after growth of T7+ on Shigella D2 371-48 often acquire a deletion in the 0.7 gene that is not necessary for the ss--phenotype. Finally, we have found a new nonessential region of the T7 chromosome that is located between 33 and 35.5% of the T7 genome length.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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