Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1977 Jun;22(3):726–733. doi: 10.1128/jvi.22.3.726-733.1977

Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3.

R F Ramig, R K Cross, B N Fields
PMCID: PMC515772  PMID: 875135

Abstract

The virus-specific double-stranded genome RNA and polypeptides present in virions and cells infected with the three mammalian reovirus serotypes have been examined by co-electrophoresis in several different polyacrylamide gel systems. The double-stranded RNA and polypeptide species previously described for type 3 Dearing were found to have corresponding species in the other serotypes examined. In each serotype several RNA and polypeptide species were found to have different electrophoretic mobilities from the corresponding RNA or polypeptide species of type 3 Dearing. The combination of electrophoretic variants among the RNAs and polypeptides of the reovirus serotypes gave electrophoretic markers in all 10 of the reovirus genes. The usefulness of these electrophoretic markers in "mapping" the reovirus genome is discussed.

Full text

PDF
726

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. Purification and properties of poliovirus double-stranded ribonucleic acid. J Mol Biol. 1966 Jul;18(3):421–428. doi: 10.1016/s0022-2836(66)80034-7. [DOI] [PubMed] [Google Scholar]
  2. Bellamy A. R., Joklik W. K. Studies on reovirus RNA. II. Characterization of reovirus messenger RNA and of the genome RNA segments from which it is transcribed. J Mol Biol. 1967 Oct 14;29(1):19–26. doi: 10.1016/0022-2836(67)90178-7. [DOI] [PubMed] [Google Scholar]
  3. Bellamy A. R., Shapiro L., August J. T., Joklik W. K. Studies on reovirus RNA. I. Characterization of reovirus genome RNA. J Mol Biol. 1967 Oct 14;29(1):1–17. doi: 10.1016/0022-2836(67)90177-5. [DOI] [PubMed] [Google Scholar]
  4. Borun T. W., Scharff M. D., Robbins E. Preparation of mammalian polyribosomes with the detergent Nonidet P-40. Biochim Biophys Acta. 1967 Nov 21;149(1):302–304. doi: 10.1016/0005-2787(67)90715-0. [DOI] [PubMed] [Google Scholar]
  5. Both G. W., Lavi S., Shatkin A. J. Synthesis of all the gene products of the reovirus genome in vivo and in vitro. Cell. 1975 Feb;4(2):173–180. doi: 10.1016/0092-8674(75)90124-5. [DOI] [PubMed] [Google Scholar]
  6. Cross R. K., Fields B. N. Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis. J Virol. 1976 Jul;19(1):162–173. doi: 10.1128/jvi.19.1.162-173.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cross R. K., Fields B. N. Temperature-sensitive mutants of reovirus type 3: evidence for aberrant mu 1 and mu 2 polypeptide species. J Virol. 1976 Jul;19(1):174–179. doi: 10.1128/jvi.19.1.174-179.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cross R. K., Fields B. N. Temperature-sensitive mutants of reovirus type 3: studies on the synthesis of viral RNA. Virology. 1972 Dec;50(3):799–809. doi: 10.1016/0042-6822(72)90434-5. [DOI] [PubMed] [Google Scholar]
  9. Cross R. K., Fields B. N. Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3. Virology. 1976 Oct 15;74(2):345–362. doi: 10.1016/0042-6822(76)90341-x. [DOI] [PubMed] [Google Scholar]
  10. De Villiers E. M. Comparison of the capsid polypeptides of various bluetongue virus serotypes. Intervirology. 1974;3(1-2):47–53. doi: 10.1159/000149741. [DOI] [PubMed] [Google Scholar]
  11. Huismans H., Howell P. G. Molecular hybridization studies on the relationships between different serotypes of bluetongue virus and on the difference between the virulent and attenuated strains of the same serotype. Onderstepoort J Vet Res. 1973 Sep;40(3):93–103. [PubMed] [Google Scholar]
  12. Krystal G., Perrault J., Graham A. F. Evidence for a glycoprotein in reovirus. Virology. 1976 Jul 15;72(2):308–321. doi: 10.1016/0042-6822(76)90160-4. [DOI] [PubMed] [Google Scholar]
  13. Krystal G., Winn P., Millward S., Sakuma S. Evidence for phosphoproteins in reovirus. Virology. 1975 Apr;64(2):505–512. doi: 10.1016/0042-6822(75)90127-0. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Martinson H. G., Lewandowski L. J. Sequence homology studies between the double-stranded RNA genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus strains 1, 2 and 3. Intervirology. 1974;4(2):91–98. doi: 10.1159/000149847. [DOI] [PubMed] [Google Scholar]
  16. McDowell M. J., Joklik W. K., Villa-Komaroff L., Lodish H. F. Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649–2653. doi: 10.1073/pnas.69.9.2649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oda K. I., Joklik W. K. Hybridization and sedimentation studies on "early" and "late" vaccinia messenger RNA. J Mol Biol. 1967 Aug 14;27(3):395–419. doi: 10.1016/0022-2836(67)90047-2. [DOI] [PubMed] [Google Scholar]
  18. Palese P., Schulman J. L. Differences in RNA patterns of influenza A viruses. J Virol. 1976 Mar;17(3):876–884. doi: 10.1128/jvi.17.3.876-884.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Palese P., Schulman J. L. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142–2146. doi: 10.1073/pnas.73.6.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pereira L., Cassai E., Honess R. W., Roizman B., Terni M., Nahmias A. Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect Immun. 1976 Jan;13(1):211–220. doi: 10.1128/iai.13.1.211-220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROSEN L. Reoviruses in animals other than man. Ann N Y Acad Sci. 1962 Nov 30;101:461–465. doi: 10.1111/j.1749-6632.1962.tb18886.x. [DOI] [PubMed] [Google Scholar]
  22. ROSEN L. Serologic grouping of reoviruses by hemagglutination-inhibition. Am J Hyg. 1960 Mar;71:242–249. doi: 10.1093/oxfordjournals.aje.a120107. [DOI] [PubMed] [Google Scholar]
  23. Ritchey M. B., Palese P., Kilbourne E. D. RNAs of influenza A, B, and C viruses. J Virol. 1976 May;18(2):738–744. doi: 10.1128/jvi.18.2.738-744.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SABIN A. B. Reoviruses. A new group of respiratory and enteric viruses formerly classified as ECHO type 10 is described. Science. 1959 Nov 20;130(3386):1387–1389. doi: 10.1126/science.130.3386.1387. [DOI] [PubMed] [Google Scholar]
  25. Shatkin A. J., Sipe J. D., Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  27. Wunner W. H., Pringle C. R. A temperature-sensitive mutant of vesicular stomatitis virus with two abnormal virus proteins. J Gen Virol. 1974 Apr;23(1):97–106. doi: 10.1099/0022-1317-23-1-97. [DOI] [PubMed] [Google Scholar]
  28. Zweerink H. J., McDowell M. J., Joklik W. K. Essential and nonessential noncapsid reovirus proteins. Virology. 1971 Sep;45(3):716–723. doi: 10.1016/0042-6822(71)90185-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES