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. 1977 Jun;22(3):750–757. doi: 10.1128/jvi.22.3.750-757.1977

Cell-free synthesis of herpes simplex virus proteins.

K J Cremer, W C Summers, R F Gesteland
PMCID: PMC515775  PMID: 195089

Abstract

Polyribosomes isolated from herpes simplex virus type I (HSV-1)-infected cells have been used to program a eucaryotic cell-free translation system. At least 10 HSV-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type HSV-infected polyribosomes. Polyribosomes prepared from thymidine kinase-negative mutants of HSV direct the synthesis of three putative nonsense termination polypeptides. HSV-specific polypeptides synthesized in vitro are precipitated with antiserum to HSV-infected cell proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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