Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Jul;23(1):74–79. doi: 10.1128/jvi.23.1.74-79.1977

Type C viral gag gene expression in chicken embryo fibroblasts and avian sarcoma virus-transformed mammalian cells.

F H Reynolds Jr, C A Hanson, S A Aaronson, J R Stephenson
PMCID: PMC515801  PMID: 196106

Abstract

Sensitive radioimmunoassays were developed for avian type C viral gag gene-coded proteins. These assays were used to examine the restriction to virus production by avian embryo cells and mammalian cells transformed by avian sarcoma viruses. The results indicate that although a high-molecular-weight primary translational product of the gag gene is expressed, its cleavage and processing are incomplete. Furthermore, analysis of intermediate cleavage products provided information regarding the order of sequences coding for the individual viral proteins within the avian type C viral gag gene.

Full text

PDF
74

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D. Tumor viruses: 1974. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1187–1200. doi: 10.1101/sqb.1974.039.01.137. [DOI] [PubMed] [Google Scholar]
  3. Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
  4. Bolognesi D. P., Ishizaki R., Hüper G., Vanaman T. C., Smith R. E. Immunological properties of avian oncornavirus polypeptides. Virology. 1975 Apr;64(2):349–357. doi: 10.1016/0042-6822(75)90111-7. [DOI] [PubMed] [Google Scholar]
  5. Chen J. H., Hanafusa H. Detection of a protein of avian leukoviruses in uninfected chick cells by radioimmunoassay. J Virol. 1974 Feb;13(2):340–346. doi: 10.1128/jvi.13.2.340-346.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chen J. H., Hayward W. S., Hanafusa H. Avian tumor virus proteins and RNA in uninfected chicken embryo cells. J Virol. 1974 Dec;14(6):1419–1429. doi: 10.1128/jvi.14.6.1419-1429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duesberg P. H., Kawai S., Wang L. H., Vogt P. K., Murphy H. M., Hanafusa H. RNA of replication-defective strains of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1569–1573. doi: 10.1073/pnas.72.4.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duesberg P. H., Vogt P. K. Differences between the ribonucleic acids of transforming and nontransforming avian tumor viruses. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1673–1680. doi: 10.1073/pnas.67.4.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eisenman R., Vogt V. M., Diggelmann H. Synthesis of avian RNA tumor virus structural proteins. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1067–1075. doi: 10.1101/sqb.1974.039.01.122. [DOI] [PubMed] [Google Scholar]
  10. Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fletcher P., Nowinski R. C., Tress E., Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. III. Avian viral proteins with group-specific antigenicity. Virology. 1975 Apr;64(2):358–366. doi: 10.1016/0042-6822(75)90112-9. [DOI] [PubMed] [Google Scholar]
  12. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hayman M. J., Vogt P. K. Subgroup-specific antigenic determinants of avian RNA tumor virls structural proteins: analysis of virus recombinants. Virology. 1976 Sep;73(2):372–380. doi: 10.1016/0042-6822(76)90398-6. [DOI] [PubMed] [Google Scholar]
  14. Herman A. C., Green R. W., Bolognesi D. P., Vanaman T. C. Comparative chemical properties of avian oncornavirus polypeptides. Virology. 1975 Apr;64(2):339–348. doi: 10.1016/0042-6822(75)90110-5. [DOI] [PubMed] [Google Scholar]
  15. Joho R. H., Billeter M. A., Weissmann C. Mapping of biological functions on RNA of avian tumor viruses: location of regions required for transformation and determination of host range. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4772–4776. doi: 10.1073/pnas.72.12.4772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kawai S., Hanafusa H. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant. Virology. 1971 Nov;46(2):470–479. doi: 10.1016/0042-6822(71)90047-x. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Martin G. S. Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature. 1970 Sep 5;227(5262):1021–1023. doi: 10.1038/2271021a0. [DOI] [PubMed] [Google Scholar]
  20. Pal B. K., Roy-Burman P. Phosphoproteins: structural components of oncornaviruses. J Virol. 1975 Mar;15(3):540–549. doi: 10.1128/jvi.15.3.540-549.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rohrschneider J. M., Diggelmann H., Ogura H., Friis R. R., Bauer H. Defective cleavage of a precursor polypeptide in a temperature-sensitive mutant of avian sarcoma virus. Virology. 1976 Nov;75(1):177–187. doi: 10.1016/0042-6822(76)90016-7. [DOI] [PubMed] [Google Scholar]
  22. SVOBODA J. Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue. Nature. 1960 Jun 18;186:980–981. doi: 10.1038/186980b0. [DOI] [PubMed] [Google Scholar]
  23. Smith E. J., Stephenson J. R., Crittenden L. B., Aaronson S. A. Avian leukosis-sarcoma virus gene expression. Noncoordinate control of group-specific antigens in virus-negative avian cells. Virology. 1976 Apr;70(2):493–501. doi: 10.1016/0042-6822(76)90290-7. [DOI] [PubMed] [Google Scholar]
  24. Stephenson J. R., Smith E. J., Crittenden L. B., Aaronson S. A. Analysis of antigenic determinants of structural polypeptides of avian type C tumor viruses. J Virol. 1975 Jul;16(1):27–33. doi: 10.1128/jvi.16.1.27-33.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stephenson J. R., Wilsnack R. E., Aaronson S. A. Radioimmunoassay for avian C-type virus group-specific antigen: detection in normal and virus-transformed cells. J Virol. 1973 Jun;11(6):893–899. doi: 10.1128/jvi.11.6.893-899.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Suni J., Vaheri A., Ruoslahti E. Radioimmunoassay of avian RNA tumor virus group-specific antigen. Intervirology. 1973;1(2):119–126. doi: 10.1159/000148838. [DOI] [PubMed] [Google Scholar]
  27. Vogt V. M., Eisenman R., Diggelmann H. Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide. J Mol Biol. 1975 Aug 15;96(3):471–493. doi: 10.1016/0022-2836(75)90174-6. [DOI] [PubMed] [Google Scholar]
  28. Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wang L. H., Duesberg P. H., Kawai S., Hanafusa H. Location of envelope-specific and sarcoma-specific oligonucleotides on RNA of Schmidt-Ruppin Rous sarcoma virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):447–451. doi: 10.1073/pnas.73.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang L. H., Duesberg P., Beemon K., Vogt P. K. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end. J Virol. 1975 Oct;16(4):1051–1070. doi: 10.1128/jvi.16.4.1051-1070.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wang L., Galehouse D., Mellon P., Duesberg P., Mason W. S., Vogt P. K. Mapping oligonucleotides of Rous sarcoma virus RNA that segregate with polymerase and group-specific antigen markers in recombinants. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3952–3956. doi: 10.1073/pnas.73.11.3952. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES