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. 1977 Jul;23(1):152–166. doi: 10.1128/jvi.23.1.152-166.1977

Oligonucleotide fingerprints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup.

J P Clewley, D H Bishop, C Y Kang, J Coffin, W M Schnitzlein, M E Reichmann, R E Shope
PMCID: PMC515811  PMID: 196099

Abstract

The relationships among the genomes of various rhabdoviruses belonging to the vesicular stomatitis virus subgroup were analyzed by an oligonucleotide fingerprinting technique. Of 10 vesicular stomatitis viruses, Indiana serotype (VSV Indiana), obtained from various sources, either no, few, or many differences were observed in the oligonucleotide fingerprints of the 42S RNA species extracted from standard B virions. Analyses of the oligonucleotides obtained from RNA extracted from three separate preparations of VSV Indiana defective T particles showed that their RNAs contain fewer oligonucleotides than the corresponding B particle RNA species. The fingerprints of RNA obtained from five VSV New Jersey serotype viruses were easily distinguished from those of the VSV Indiana isolates. Three of the VSV New Jersey RNA fingerprints were similar to each other but quite different from those of the other two viruses. The RNA fingerprints of two Chandipura virus isolates (one obtained from India and one from Nigeria) were also unique, whereas the fingerprint of Cocal virus RNA was unlike that of the serologically related VSV Indiana.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaslestad H. G., Clark H. F., Bishop D. H., Koprowski H. Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. J Virol. 1971 Jun;7(6):726–735. doi: 10.1128/jvi.7.6.726-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRADISH C. J., BROOKSBY J. B., DILLON J. F., Jr Biophysical studies of the virus system of vesicular stomatitis. J Gen Microbiol. 1956 Apr;14(2):290–314. doi: 10.1099/00221287-14-2-290. [DOI] [PubMed] [Google Scholar]
  3. Bishop D. H., Emerson S. U., Flamand A. Reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (Indiana serotype), Chandipura, vesicular stomatitis (New Jersey serotype), and Cocal viruses. J Virol. 1974 Jul;14(1):139–144. doi: 10.1128/jvi.14.1.139-144.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop D. H., Roy P. Properties of the product synthesized by vesicular stomatitis virus particles. J Mol Biol. 1971 Jun 28;58(3):799–814. doi: 10.1016/0022-2836(71)90041-6. [DOI] [PubMed] [Google Scholar]
  5. CHOPPIN P. W. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE. Virology. 1964 Jun;23:224–233. doi: 10.1016/0042-6822(64)90286-7. [DOI] [PubMed] [Google Scholar]
  6. COOPER P. D., BELLETT A. J. A transmissible interfering component of vesicular stomatitis virus preparations. J Gen Microbiol. 1959 Dec;21:485–497. doi: 10.1099/00221287-21-3-485. [DOI] [PubMed] [Google Scholar]
  7. Cartwright B., Brown F. Serological relationships between different strains of vesicular stomatis virus. J Gen Virol. 1972 Sep;16(3):391–398. doi: 10.1099/0022-1317-16-3-391. [DOI] [PubMed] [Google Scholar]
  8. Clewley J., Gentsch J., Bishop D. H. Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. J Virol. 1977 May;22(2):459–468. doi: 10.1128/jvi.22.2.459-468.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crick J., Brown F. Interference as a measure of cross-relationships in the vesicular stomatitis group of rhabdo viruses. J Gen Virol. 1973 Jan;18(1):79–82. doi: 10.1099/0022-1317-18-1-79. [DOI] [PubMed] [Google Scholar]
  10. Federer K. E., Burrows R., Brooksby J. B. Vesicular stomatitis virus--the relationship between some strains of the Indiana serotype. Res Vet Sci. 1967 Jan;8(1):103–117. [PubMed] [Google Scholar]
  11. HANSON R. P. The natural history of vesicular stomatitis. Bacteriol Rev. 1952 Sep;16(3):179–204. doi: 10.1128/br.16.3.179-204.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanson R. P. Discussion of the natural history of vesicular stomatitis. Am J Epidemiol. 1968 Mar;87(2):264–266. doi: 10.1093/oxfordjournals.aje.a120815. [DOI] [PubMed] [Google Scholar]
  13. Huang A. S., Wagner R. R. Defective T particles of vesicular stomatitis virus. II. Biologic role in homologous interference. Virology. 1966 Oct;30(2):173–181. doi: 10.1016/0042-6822(66)90093-6. [DOI] [PubMed] [Google Scholar]
  14. JENNEY E. W., MOTT L. O. Serologic studies with the virus of vesicular stomatitis. II. Typing of vesicular stomatitis convalescent serum by direct complement fixation. Am J Vet Res. 1963 Jul;24:874–879. [PubMed] [Google Scholar]
  15. JONKERS A. H., SHOPE R. E., AITKEN T. H., SPENCE L. COCAL VIRUS, A NEW AGENT IN TRINIDAD RELATED TO VESICULAR STOMATITIS VIRUS, TYPE INDIANA. Am J Vet Res. 1964 Jan;25:236–242. [PubMed] [Google Scholar]
  16. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. J Virol. 1969 Apr;3(4):404–413. doi: 10.1128/jvi.3.4.404-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. J Virol. 1970 Jul;6(1):20–27. doi: 10.1128/jvi.6.1.20-27.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Leamnson R. N., Reichmann M. E. The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons. J Mol Biol. 1974 Jan 5;85(4):551–568. doi: 10.1016/0022-2836(74)90315-5. [DOI] [PubMed] [Google Scholar]
  19. Nakai T., Howatson A. F. The fine structure of vesicular stomatitis virus. Virology. 1968 Jun;35(2):268–281. doi: 10.1016/0042-6822(68)90267-5. [DOI] [PubMed] [Google Scholar]
  20. Obijeski J. F., Marchenko A. T., Bishop D. H., Cann B. W., Murphy F. A. Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. J Gen Virol. 1974 Jan;22(1):21–33. doi: 10.1099/0022-1317-22-1-21. [DOI] [PubMed] [Google Scholar]
  21. PAUCKER K., SKURSKA Z., HENLE W. Quantitative studies on viral interference in suspended L cells. I. Growth characteristics and interfering activities of vesicular stomatitis, Newcastle disease, and influenza A viruses. Virology. 1962 Jun;17:301–311. doi: 10.1016/0042-6822(62)90121-6. [DOI] [PubMed] [Google Scholar]
  22. Perrault J. Cross-linked double-stranded RNA from a defective vesicular stomatitis virus particle. Virology. 1976 Apr;70(2):360–371. doi: 10.1016/0042-6822(76)90278-6. [DOI] [PubMed] [Google Scholar]
  23. Petric M., Prevec L. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970 Aug;41(4):615–630. doi: 10.1016/0042-6822(70)90427-7. [DOI] [PubMed] [Google Scholar]
  24. Pringle C. R., Duncan I. B., Stevenson M. Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. J Virol. 1971 Dec;8(6):836–841. doi: 10.1128/jvi.8.6.836-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pringle C. R. Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethyl methane sulfonate. J Virol. 1970 May;5(5):559–567. doi: 10.1128/jvi.5.5.559-567.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Pringle C. R., Wunner W. H. Genetic and physiological properties of temperature-sensitive mutants of Cocal virus. J Virol. 1973 Oct;12(4):677–683. doi: 10.1128/jvi.12.4.677-683.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Reichmann M. E., Pringle C. R., Follett E. A. Defective particles in BHK cells infected with temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1971 Aug;8(2):154–160. doi: 10.1128/jvi.8.2.154-160.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Repik P., Bishop D. H. Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle. J Virol. 1973 Nov;12(5):969–983. doi: 10.1128/jvi.12.5.969-983.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Repik P., Flamand A., Bishop D. H. Synthesis of RNA by mutants of vesicular stomatitis virus (Indiana serotype) and the ability of wild-type VSV New Jersey to complement the VSV Indiana ts G I-114 transcription defect. J Virol. 1976 Oct;20(1):157–169. doi: 10.1128/jvi.20.1.157-169.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Repik P., Flamand A., Clark H. F., Obijeski J. F., Roy P., Bishop D. H. Detection of homologous RNA sequences among six rhabdovirus genomes. J Virol. 1974 Jan;13(1):250–252. doi: 10.1128/jvi.13.1.250-252.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Roy P., Bishop D. H. Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid. J Virol. 1972 Jun;9(6):946–955. doi: 10.1128/jvi.9.6.946-955.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Roy P., Repik P., Hefti E., Bishop D. H. Complementary RNA species isolated from vesicular stomatitis (HR strain) defective virions. J Virol. 1973 Jun;11(6):915–925. doi: 10.1128/jvi.11.6.915-925.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schnitzlein W. M., Reichmann M. E. The size and the cistronic origin of defective vesicular stomatitis virus particle RNAs in relation to homotypic and heterotypic interference. J Mol Biol. 1976 Mar 5;101(3):307–325. doi: 10.1016/0022-2836(76)90150-9. [DOI] [PubMed] [Google Scholar]
  34. Simpson R. W., Hauser R. E. Structural components of vesicular stomatitis virus. Virology. 1966 Aug;29(4):654–667. doi: 10.1016/0042-6822(66)90289-3. [DOI] [PubMed] [Google Scholar]
  35. Stamminger G., Lazzarini R. A. Analysis of the RNA of defective VSV particles. Cell. 1974 Sep;3(1):85–93. doi: 10.1016/0092-8674(74)90044-0. [DOI] [PubMed] [Google Scholar]
  36. Sudia W. D., Fields B. N., Calisher C. H. The isolation of vesiculay stomatitis virus (Indiana strain) and other viruses from mosquitoes in New Mexico, 1965. Am J Epidemiol. 1967 Nov;86(3):598–602. doi: 10.1093/oxfordjournals.aje.a120769. [DOI] [PubMed] [Google Scholar]
  37. Sudia W. D., Lord R. D., Newhouse V. F., Miller D. L., Kissling R. E. Vector-host studies of an epizootic of Venezuelan equine encephalomyelitis in Guatemala, 1969. Am J Epidemiol. 1971 Feb;93(2):137–143. doi: 10.1093/oxfordjournals.aje.a121234. [DOI] [PubMed] [Google Scholar]
  38. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  39. de Wachter R., Fiers W. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972 Sep;49(1):184–197. doi: 10.1016/0003-2697(72)90257-6. [DOI] [PubMed] [Google Scholar]

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