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. 1977 Aug;23(2):286–293. doi: 10.1128/jvi.23.2.286-293.1977

Oligosaccharides of the glycoprotein of rabies virus.

B Dietzschold
PMCID: PMC515830  PMID: 886651

Abstract

The number of oligosaccharide side chains on rabies virus glycoprotein (G-protein) was investigated. Analysis of glycopeptides obtained by protease digestion of desialated G-protein revealed three discrete glycopeptides. Comparison of the protease digestion products from desialated and from untreated G-protein indicated a heterogeneity among the glycopeptides in the sialic acid content. Two major tryptic glycopeptides were isolated from desialated rabies virus G-protein and analyzed after protease digestion; one contained two oligosaccharide side chains and the other contained a single oligosaccharide side chain.

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Selected References

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  1. Atanasiu P., Tsiang H., Perrin P., Favre S. Analyse du pouvoir immunogène et protecteur de la glycoprotéine extraite du virus rabique: comparison de préparations purifiées par des techniques différentes et résultats. Ann Microbiol (Paris) 1976 Aug-Sep;127B(2):257–267. [PubMed] [Google Scholar]
  2. Burge B. W., Huang A. S. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. J Virol. 1970 Aug;6(2):176–182. doi: 10.1128/jvi.6.2.176-182.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burge B. W., Strauss J. H., Jr Glycopeptides of the membrane glycoprotein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):449–466. doi: 10.1016/0022-2836(70)90314-1. [DOI] [PubMed] [Google Scholar]
  4. Cooper P. D., Summers D. F., Maizel J. V. Evidence for ambiguity in the posttranslational cleavage of poliovirus proteins. Virology. 1970 Jul;41(3):408–418. doi: 10.1016/0042-6822(70)90161-3. [DOI] [PubMed] [Google Scholar]
  5. Etchison J. R., Holland J. J. Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus. Virology. 1974 Jul;60(1):217–229. doi: 10.1016/0042-6822(74)90379-1. [DOI] [PubMed] [Google Scholar]
  6. Johnson I., Clamp J. R. The oligosaccharide units of a human type L immunoglobulin M (Macroglobulin). Biochem J. 1971 Aug;123(5):739–745. doi: 10.1042/bj1230739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keegstra K., Sefton B., Burke D. Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides. J Virol. 1975 Sep;16(3):613–620. doi: 10.1128/jvi.16.3.613-620.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lai M. M., Duesberg P. H. Differences between the envelope glycoproteins and glycopeptides of avian tumor viruses released from transformed and from nontransformed cells. Virology. 1972 Nov;50(2):359–372. doi: 10.1016/0042-6822(72)90387-x. [DOI] [PubMed] [Google Scholar]
  10. Robertson J. S., Etchison J. R., Summers D. F. Glycosylation sites of vesicular stomatitis virus glycoprotein. J Virol. 1976 Sep;19(3):871–878. doi: 10.1128/jvi.19.3.871-878.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SPIRO R. G. THE CARBOHYDRATE UNITS OF THYROGLOBULIN. J Biol Chem. 1965 Apr;240:1603–1610. [PubMed] [Google Scholar]
  12. STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
  13. Schlumberger H. D., Schneider L. G., Kulas H. P., Diringer H. Gross chemical composition of strain Flury HEP rabies virus. Z Naturforsch C. 1973 Jan-Feb;28(1):103–104. [PubMed] [Google Scholar]
  14. Sefton B. M., Keegstra K. Glycoproteins of Sindbis virus: priliminary characterization of the oligosaccharides. J Virol. 1974 Sep;14(3):522–530. doi: 10.1128/jvi.14.3.522-530.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sokol F., Stancek D., Koprowski H. Structural proteins of rabies virus. J Virol. 1971 Feb;7(2):241–249. doi: 10.1128/jvi.7.2.241-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spiro R. G. Glycoproteins. Adv Protein Chem. 1973;27:349–467. doi: 10.1016/s0065-3233(08)60451-9. [DOI] [PubMed] [Google Scholar]
  17. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  18. Wiktor T. J., Dietzschold B., Leamnson R. N., Koprowski H. Induction and biological properties of defective interfering particles of rabies virus. J Virol. 1977 Feb;21(2):626–635. doi: 10.1128/jvi.21.2.626-635.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wiktor T. J., György E., Schlumberger D., Sokol F., Koprowski H. Antigenic properties of rabies virus components. J Immunol. 1973 Jan;110(1):269–276. [PubMed] [Google Scholar]

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