Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1977 Aug;23(2):315–322. doi: 10.1128/jvi.23.2.315-322.1977

Identification of the Gross cell surface antigen associated with murine leukemia virus-infected cells.

J Ledbetter, R C Nowinski
PMCID: PMC515833  PMID: 69726

Abstract

The Gross cell surface antigen (GCSA) is produced by cells that are either exogenously infected with murine leukemia virus (MuLV) or are expressing endogenous MuLV genomes. In immune precipitation assays, GCSA was resolved into two serologically distinct 85,000- and 95,000-dalton viral proteins. These antigenic components are glycosylated forms of the polyprotein precursors of the MuLV internal structural proteins.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Boyse E. A., Old L. J., De Harven E., Hämmerling U., Wood H. A. G (Gross) and H-2 cell-surface antigens: location on Gross leukemia cells by electron microscopy with visually labeled antibody. Proc Natl Acad Sci U S A. 1970 Mar;65(3):569–576. doi: 10.1073/pnas.65.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  3. Fleissner E., Tress E. Chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by Rous sarcoma virus. J Virol. 1973 Feb;11(2):250–262. doi: 10.1128/jvi.11.2.250-262.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ihle J. N., Hanna M. G., Jr, Roberson L. E., Kenney F. T. Autogenous immunity to endogenous RNA tumor virus. Identification of antibody reactivity to select viral antigens. J Exp Med. 1974 Jun 1;139(6):1568–1581. doi: 10.1084/jem.139.6.1568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ihle J. N., Yurconic M., Jr, Hanna M. G., Jr Autogenous immunity to endogenous RNA tumor virus. Radioimmune precipitation assay of mouse serum antibody levels. J Exp Med. 1973 Jul 1;138(1):194–208. doi: 10.1084/jem.138.1.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  8. Knight R. A., Mitchison N. A., Shellam G. R. Studies on a gross-virus-induced lymphoma in the rat. II. The role of cell-membrane-associated and serum P30 antigen in the antibody and cell-mediated response. Int J Cancer. 1975 Mar 15;15(3):417–428. doi: 10.1002/ijc.2910150308. [DOI] [PubMed] [Google Scholar]
  9. Ledbetter J., Nowinski R. C., Emery S. Viral proteins expressed on the surface of murine leukemia cells. J Virol. 1977 Apr;22(1):65–73. doi: 10.1128/jvi.22.1.65-73.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nowinski R. C., Kaehler S. L., Burgess R. R. Immune response in the mouse to endogenous leukemia viruses. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1123–1128. doi: 10.1101/sqb.1974.039.01.128. [DOI] [PubMed] [Google Scholar]
  11. Nowinski R. C., Watson A. Immune response of the mouse to the major core protein (p30) of ecotropic leukemia viruses. J Immunol. 1976 Aug;117(2):693–696. [PubMed] [Google Scholar]
  12. O'Donnell P. V., Stockert E. Induction of GIX antigen and gross cell surface antigen after infection by ecotropic and xenotropic murine leukemia viruses in vitro. J Virol. 1976 Dec;20(3):545–554. doi: 10.1128/jvi.20.3.545-554.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OLD L. J., BOYSE E. A., STOCKERT E. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS. Nature. 1964 Feb 22;201:777–779. doi: 10.1038/201777a0. [DOI] [PubMed] [Google Scholar]
  14. Old L. J., Boyse E. A., Stockert E. The G (Gross) leukemia antigen. Cancer Res. 1965 Jul;25(6):813–819. [PubMed] [Google Scholar]
  15. Snyder H. W., Jr, Stockert E., Fleissner E. Characterization of molecular species carrying gross cell surface antigen. J Virol. 1977 Aug;23(2):302–314. doi: 10.1128/jvi.23.2.302-314.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stephenson J. R., Peters R. L., Hino S., Donahoe R. M., Long L. K., Aaronson S. A., Kelloff G. J. Natural immunity in mice to structural polypeptides of endogenous type C RNA viruses. J Virol. 1976 Sep;19(3):890–898. doi: 10.1128/jvi.19.3.890-898.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tung J. S., Fleissner E., Vitetta E. S., Boyse E. A. Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs. absence of GIX antigen. J Exp Med. 1975 Aug 1;142(2):518–523. doi: 10.1084/jem.142.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tung J. S., Yoshiki T., Fleissner E. A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells. Cell. 1976 Dec;9(4 Pt 1):573–578. doi: 10.1016/0092-8674(76)90039-8. [DOI] [PubMed] [Google Scholar]
  19. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES