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. 1977 Sep;23(3):669–678. doi: 10.1128/jvi.23.3.669-678.1977

HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.

R Wittek, A Menna, D Schümperli, S Stoffel, H K Müller, R Wyler
PMCID: PMC515878  PMID: 197263

Abstract

The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.

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