Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Sep;23(3):692–699. doi: 10.1128/jvi.23.3.692-699.1977

Simian virus 40-specific ribosome-binding proteins induced by a nondefective adenovirus 2-simian virus 40 hybrid.

G Jay, F T Jay, R M Friedman, A S Levine
PMCID: PMC515881  PMID: 197266

Abstract

We have studied the intracellular distribution of the two simian virus 40-specific proteins, with apparent molecular weights of 56,000 and 42,000, detectable in human KB cells infected by a nondefective adenovirus 2-simian virus 40 hybrid, Ad2+ND2. After a 20-min pulse of [35S]methionine, about two-thirds of the newly synthesized 56K protein and one-third of the 42K protein were found localized on the plasma membrane. The remainder of each protein was found in the cytoplasm, whereas the nuclear fraction was virtually free of either component. A significant portion of both proteins present in the cytoplasmic fraction was complexed to the 40S ribosomal subunits and was not removed by treatment with 0.5 M KCl. Moreover, the portion that was found free in the cytoplasm could bind preferentially and quantitatively to purified 40S ribosomes in vitro, leading us to propose that these simian virus 40 proteins may act as translational control elements in cells.

Full text

PDF
692

Images in this article

694Image
on p.694
695Image
on p.695
696Image
on p.696
697Image
on p.697

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLACK P. H., ROWE W. P., TURNER H. C., HUEBNER R. J. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Dec;50:1148–1156. doi: 10.1073/pnas.50.6.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baum S. G., Horwitz M. S., Maizel J. V., Jr Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. J Virol. 1972 Aug;10(2):211–219. doi: 10.1128/jvi.10.2.211-219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deppert W., Walter G. Simian virus to (SV40) tumor-specific proteins in nucleus and plasma membrane of HeLa cells infected by adenovirus 2-SV40 hybrid virus Ad2+ND2. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2505–2509. doi: 10.1073/pnas.73.7.2505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fox R. I., Baum S. G. Synthesis of viral ribonucleic acid during restricted adenovirus infection. J Virol. 1972 Aug;10(2):220–227. doi: 10.1128/jvi.10.2.220-227.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Freienstein C., Blobel G. Nonribosomal proteins associated with eukaryotic native small ribosomal subunits. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3392–3396. doi: 10.1073/pnas.72.9.3392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Girardi A. J., Defendi V. Induction of SV40 transplantation antigen (TrAg) during the lytic cycle. Virology. 1970 Nov;42(3):688–698. doi: 10.1016/0042-6822(70)90314-4. [DOI] [PubMed] [Google Scholar]
  8. Grodzicker T., Anderson C., Sharp P. A., Sambrook J. Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. I. Host range mutants of Ad2+ND1. J Virol. 1974 Jun;13(6):1237–1244. doi: 10.1128/jvi.13.6.1237-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horwitz M. S., Scharff M. D., Maizel J. V., Jr Synthesis and assembly of adenovirus 2. I. Polypeptide synthesis, assembly of capsomeres, and morphogenesis of the virion. Virology. 1969 Dec;39(4):682–694. doi: 10.1016/0042-6822(69)90006-3. [DOI] [PubMed] [Google Scholar]
  10. Jerkofsky M. Enhancement of the replication of human adenovirus in simian cells by a series of temperature-sensitive mutants of simian virus 40. Virology. 1975 Jun;65(2):579–582. doi: 10.1016/0042-6822(75)90063-x. [DOI] [PubMed] [Google Scholar]
  11. KHERA K. S., ASHKENAZI A., RAPP F., MELNICK J. L. IMMUNITY IN HAMSTERS TO CELLS TRANSFORMED IN VITRO AND IN VIVO BY SV40. TESTS FOR ANTIGENIC RELATIONSHIP AMONG THE PAPOVAVIRUSES. J Immunol. 1963 Nov;91:604–613. [PubMed] [Google Scholar]
  12. Kimura G. Genetic evidence for SV40 gene function in enhancement of replication of human adenovirus in simian cells. Nature. 1974 Apr 12;248(449):590–592. doi: 10.1038/248590a0. [DOI] [PubMed] [Google Scholar]
  13. Kit S. Viral-induced enzymes and the problem of viral oncogenesis. Adv Cancer Res. 1968;11:73–221. doi: 10.1016/s0065-230x(08)60388-7. [DOI] [PubMed] [Google Scholar]
  14. Klessig D. F., Anderson C. W. Block to multiplication of adenovirus serotype 2 in monkey cells. J Virol. 1975 Dec;16(6):1650–1668. doi: 10.1128/jvi.16.6.1650-1668.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  17. Levine A. S., Levin M. J., Oxman M. N., Lewis A. M., Jr Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses. J Virol. 1973 May;11(5):672–681. doi: 10.1128/jvi.11.5.672-681.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lewis A. M., Jr, Levine A. S., Crumpacker C. S., Levin M. J., Samaha R. J., Henry P. H. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties. J Virol. 1973 May;11(5):655–664. doi: 10.1128/jvi.11.5.655-664.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewis A. M., Jr, Rowe W. P. Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. J Virol. 1971 Feb;7(2):189–197. doi: 10.1128/jvi.7.2.189-197.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morrow J. F., Berg P., Kelly T. J., Jr, Lewis A. M., Jr Mapping of simian virus 40 early functions on the viral chromosome. J Virol. 1973 Sep;12(3):653–658. doi: 10.1128/jvi.12.3.653-658.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nakajima K., Ishitsuka H., Oda K. An SV40-induced initiation factor for protein synthesis concerned with the regulation of permissiveness. Nature. 1974 Dec 20;252(5485):649–653. doi: 10.1038/252649a0. [DOI] [PubMed] [Google Scholar]
  22. Nakajima K., Oda K. The alteration of ribosomes for mRNA selection concerned with adenovirus growth in SV40-infected simian cells. Virology. 1975 Sep;67(1):85–93. doi: 10.1016/0042-6822(75)90406-7. [DOI] [PubMed] [Google Scholar]
  23. O'Farrell P. Z., Gold L. M., Huang W. M. The identification of prereplicative bacteriophage T4 proteins. J Biol Chem. 1973 Aug 10;248(15):5499–5501. [PubMed] [Google Scholar]
  24. Pettersson U., Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. doi: 10.1016/0022-2836(73)90164-2. [DOI] [PubMed] [Google Scholar]
  25. RABSON A. S., O'CONOR G. T., BEREZESKY I. K., PAUL F. J. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40. Proc Soc Exp Biol Med. 1964 May;116:187–190. doi: 10.3181/00379727-116-29197. [DOI] [PubMed] [Google Scholar]
  26. RAPP F., KITAHARA T., BUTEL J. S., MELNICK J. L. SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40). Proc Natl Acad Sci U S A. 1964 Nov;52:1138–1142. doi: 10.1073/pnas.52.5.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Russell W. C., Skehel J. J. The polypeptides of adenovirus-infected cells. J Gen Virol. 1972 Apr;15(1):45–57. doi: 10.1099/0022-1317-15-1-45. [DOI] [PubMed] [Google Scholar]
  28. TEVETHIA S. S., KATZ M., RAPP F. NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1965 Jul;119:896–901. doi: 10.3181/00379727-119-30330. [DOI] [PubMed] [Google Scholar]
  29. Velicer L. F., Ginsberg H. S. Synthesis, transport, and morphogenesis of type adenovirus capsid proteins. J Virol. 1970 Mar;5(3):338–352. doi: 10.1128/jvi.5.3.338-352.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Walter G., Martin H. Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. J Virol. 1975 Nov;16(5):1236–1247. doi: 10.1128/jvi.16.5.1236-1247.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. van der Vliet P. C., Levine A. J. DNA-binding proteins specific for cells infected by adenovirus. Nat New Biol. 1973 Dec 12;246(154):170–174. doi: 10.1038/newbio246170a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES