Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1977 Sep;23(3):820–824. doi: 10.1128/jvi.23.3.820-824.1977

Antibody-independent detection of virus-specific glycoprotein synthesis is oncornavirus-infected cells.

J M England, B Dietzschold, M S Halpern
PMCID: PMC515894  PMID: 197273

Abstract

A method is described for detecting the synthesis of avian and murine oncornavirus-specific glycoproteins without the use of antibodies raised against viral structural proteins. As applied to cells infected with avian tumor virus, the method served to resolve pr90, the precursor of the major envelope glycoprotein. A virus-specific glycoprotein of about 85,000 daltons, which has several properties expected to a precursor to gp69/71, was detected in cells infected with murine leukemia virus.

Full text

PDF
820

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arcement L. J., Karshin W. L., Naso R. B., Jamjoom G., Arlinghaus R. B. Biosynthesis of Rauscher leukemia viral proteins: presence of p30 and envelope p15 sequences in precursor polypeptides. Virology. 1976 Feb;69(2):763–774. doi: 10.1016/0042-6822(76)90504-3. [DOI] [PubMed] [Google Scholar]
  2. England J. M., Bolognesi D. P., Dietzschold B., Halpern M. S. Evidence that a precursor glycoprotein is cleaved to yield the major glycoprotein of avian tumor virus. J Virol. 1977 Feb;21(2):810–814. doi: 10.1128/jvi.21.2.810-814.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Famulari N. G., Buchhagen D. L., Klenk H. D., Fleissner E. Presence of murine leukemia virus envelope proteins gp70 and p15(E) in a common polyprotein of infected cells. J Virol. 1976 Nov;20(2):501–508. doi: 10.1128/jvi.20.2.501-508.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Halpern M. S., Bolognesi D. P., Lewandowski L. J. Isolation of the major viral glycoprotein and a putative precursor from cells transformed by avian sarcoma viruses. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2342–2346. doi: 10.1073/pnas.71.6.2342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Knipe D. M., Lodish H. F., Baltimore D. Localization of two cellular forms of the vesicular stomatitis viral glycoprotein. J Virol. 1977 Mar;21(3):1121–1127. doi: 10.1128/jvi.21.3.1121-1127.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Leamnson R. N., Shander M. H., Halpern M. S. A structural protein complex in Moloney leukemia virus. Virology. 1977 Jan;76(1):437–439. doi: 10.1016/0042-6822(77)90318-x. [DOI] [PubMed] [Google Scholar]
  8. Madore H. P., England J. M. Rabies virus protein synthesis in infected BHK-21 cells. J Virol. 1977 Apr;22(1):102–112. doi: 10.1128/jvi.22.1.102-112.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tung J. S., Yoshiki T., Fleissner E. A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells. Cell. 1976 Dec;9(4 Pt 1):573–578. doi: 10.1016/0092-8674(76)90039-8. [DOI] [PubMed] [Google Scholar]
  10. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES