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. 1991 May 1;88(9):4050–4053. doi: 10.1073/pnas.88.9.4050

Acquisition and loss of a neuronal Ca2+/calmodulin-dependent protein kinase during neuronal differentiation.

K F Jensen 1, C A Ohmstede 1, R S Fisher 1, J K Olin 1, N Sahyoun 1
PMCID: PMC51591  PMID: 2023954

Abstract

Calcium ions play a critical role in neural development. Insights into the ontogeny of Ca(2+)-signaling pathways were gained by investigating the developmental expression of granule cell-enriched Ca2+/calmodulin-dependent protein kinase (CaM kinase-Gr) in the cerebellum and hippocampus of the rat. Neurons of these brain regions displayed characteristic schedules by which they acquired and lost CaM kinase-Gr during differentiation. In the cerebellum, granule cells did not begin to express CaM kinase-Gr until after birth when they migrated into the granule cell layer, and this expression persisted in the adult. Purkinje cells expressed CaM kinase-Gr prenatally and lost this expression by postnatal day 14. In contrast, the granule and pyramidal cells of the hippocampus expressed the enzyme prenatally and in the adult. Moreover, CaM kinase-Gr was localized to the processes and nuclei of developing neurons. This subcellular localization together with the scheduled expression of CaM kinase-Gr can serve to regulate a developing neuron's sensitivity to Ca2+ at different subcellular levels.

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Selected References

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