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Journal of Virology logoLink to Journal of Virology
. 1977 Oct;24(1):121–134. doi: 10.1128/jvi.24.1.121-134.1977

Localization of minor protein components of the head of bacteriophage T4.

L Müller-Salamin, L Onorato, M K Showe
PMCID: PMC515916  PMID: 904022

Abstract

The bacteriophage T4 capsid contains a number of minor proteins that are required for head assembly but whose detailed function and position in the head are unknown. We have found that by systematically varying the conditions of extraction, some of these minor proteins can be removed while the main capsid structure is left substantially intact. Electron microscopic examination of the residual capsids showed that the extraction of the product of gene 20 is correlated with the loss of a plug that distinguishes one vertex position (presumably the tail attachment site) from the others. Extraction of the product of gene 24 is correlated with the loss of the other 11 (nonproximal) vertexes of the capsid. We further show that antibody to P24 binds specifically to the nonproximal vertexes of both T4 preheads and T4 phages. On the basis of our findings, we suggest that P20 is located at or near the tail attachment site of the capsid, whereas P24 forms the 11 nonproximal vertexes of preheads and P24 forms the nonproximal vertexes of the mature head.

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Selected References

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  1. Aebi U., Bijlenga R., v d Broek J., v d Broek H., Eiserling F., Kellenberger C., Kellenberger E., Mesyanzhinov V., Müller L., Showe M. The transformation of tau particles into T4 heads. II. Transformations of the surface lattice and related observations on form determination. J Supramol Struct. 1974;2(2-4):253–275. doi: 10.1002/jss.400020218. [DOI] [PubMed] [Google Scholar]
  2. Bijlenga R. K., Aebi U., Kellenberger E. Properties and structure of a gene 24-controlled T4 giant phage. J Mol Biol. 1976 May 25;103(3):469–498. doi: 10.1016/0022-2836(76)90213-8. [DOI] [PubMed] [Google Scholar]
  3. Bijlenga R. K., Scraba D., Kellenberger E. Studies on the morphopoiesis of the head of T-even phage. IX. Gamma-particles: their morphology, kinetics of appearance and possible precursor function. Virology. 1973 Nov;56(1):250–267. doi: 10.1016/0042-6822(73)90304-8. [DOI] [PubMed] [Google Scholar]
  4. Black L. W. Ahmad-Zadeh C,+AHMADAAZADEH C: Internal proteins of bacteriophage T4D: their characterization and relation to head structure and assembly. J Mol Biol. 1971 Apr 14;57(1):71–92. doi: 10.1016/0022-2836(71)90120-3. [DOI] [PubMed] [Google Scholar]
  5. Boy de la Tour E., Kellenberger E. Aberrant forms of the T-even phage head. Virology. 1965 Oct;27(2):222–225. doi: 10.1016/0042-6822(65)90163-7. [DOI] [PubMed] [Google Scholar]
  6. Branton D., Klug A. Capsid geometry of bacteriophage T2: a freeze-etching study. J Mol Biol. 1975 Mar 15;92(4):559–565. doi: 10.1016/0022-2836(75)90309-5. [DOI] [PubMed] [Google Scholar]
  7. Coppo A., Manzi A., Pulitzer J. F., Takahashi H. Abortive bacteriophage T4 head assembly in mutants of Escherichia coli. J Mol Biol. 1973 May 5;76(1):61–87. doi: 10.1016/0022-2836(73)90081-8. [DOI] [PubMed] [Google Scholar]
  8. Dickson R. C., Barnes S. L., Eiserling F. A. Structural proteins of bacteriophage T4. J Mol Biol. 1970 Nov 14;53(3):461–474. doi: 10.1016/0022-2836(70)90077-x. [DOI] [PubMed] [Google Scholar]
  9. FINCH J. T., KLUG A., STRETTON A. O. THE STRUCTURE OF THE "POLYHEADS" OF T4 BACTERIOPHAGE. J Mol Biol. 1964 Dec;10:570–575. doi: 10.1016/s0022-2836(64)80082-6. [DOI] [PubMed] [Google Scholar]
  10. Forrest G. L., Cummings D. J. Head proteins from T-even bacteriophage. I. Molecular weight characterization. J Virol. 1970 Mar;5(3):398–405. doi: 10.1128/jvi.5.3.398-405.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Horvitz H. R. Bacteriophage T4 mutants deficient in alteration and modification of the Escherichia coli RNA polymerase. J Mol Biol. 1974 Dec 25;90(4):739–750. doi: 10.1016/0022-2836(74)90537-3. [DOI] [PubMed] [Google Scholar]
  12. Horvitz H. R. Control by bacteriophage T4 of two sequential phosphorylations of the alpha subunit of Escherichia coli RNA polymerase. J Mol Biol. 1974 Dec 25;90(4):727–738. doi: 10.1016/0022-2836(74)90536-1. [DOI] [PubMed] [Google Scholar]
  13. Ishii T., Yanagida M. Molecular organization of the shell of the Teven bacteriophage head. J Mol Biol. 1975 Oct 5;97(4):655–660. doi: 10.1016/s0022-2836(75)80065-9. [DOI] [PubMed] [Google Scholar]
  14. Kellenberger E., Der Kamp C. K.-V. On a modification of the gene product P23 according to its use as subunit of either normal capsids of phage T4 or of polyheads. FEBS Lett. 1970 Jun 1;8(3):140–144. doi: 10.1016/0014-5793(70)80247-2. [DOI] [PubMed] [Google Scholar]
  15. Kellenberger E., Eiserling F. A., Boy de la Tour E. Studies on the morphopoiesis of the head of phage T-even. 3. The cores of head-related structures. J Ultrastruct Res. 1967 Dec 12;21(3):335–360. doi: 10.1016/s0022-5320(67)80099-6. [DOI] [PubMed] [Google Scholar]
  16. Kellenberger E. Studies on the morphopoiesis of the head of phage T-even. V. The components of the T4 capsid and of other, capsid-related structures. Virology. 1968 Mar;34(3):549–561. doi: 10.1016/0042-6822(68)90074-3. [DOI] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  19. Laemmli U. K., Mölbert E., Showe M., Kellenberger E. Form-determining function of the genes required for the assembly of the head of bacteriophage T4. J Mol Biol. 1970 Apr 14;49(1):99–113. doi: 10.1016/0022-2836(70)90379-7. [DOI] [PubMed] [Google Scholar]
  20. Leibo S. P., Mazur P. Freezing of bacteriophage T4: temperature and rate effects as a function of salt concentration. Virology. 1969 Aug;38(4):558–566. doi: 10.1016/0042-6822(69)90176-7. [DOI] [PubMed] [Google Scholar]
  21. Moody M. F. The shape of the T-even bacteriophage head. Virology. 1965 Aug;26(4):567–576. doi: 10.1016/0042-6822(65)90319-3. [DOI] [PubMed] [Google Scholar]
  22. Onorato L., Showe M. K. Gene 21 protein-dependent proteolysis in vitro of purified gene 22 product of bacteriophage T4. J Mol Biol. 1975 Mar 5;92(3):395–412. doi: 10.1016/0022-2836(75)90288-0. [DOI] [PubMed] [Google Scholar]
  23. Paulson J. R., Lazaroff S., Laemmli U. K. Head length determination in bacteriophage T4: the role of the core protein P22. J Mol Biol. 1976 May 5;103(1):155–174. doi: 10.1016/0022-2836(76)90057-7. [DOI] [PubMed] [Google Scholar]
  24. Reid M. S., Bieleski R. L. A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis. Anal Biochem. 1968 Mar;22(3):374–381. doi: 10.1016/0003-2697(68)90278-9. [DOI] [PubMed] [Google Scholar]
  25. Showe M. K., Black L. W. Assembly core of bacteriophage T4: an intermediate in head formation. Nat New Biol. 1973 Mar 21;242(116):70–75. doi: 10.1038/newbio242070a0. [DOI] [PubMed] [Google Scholar]
  26. Showe M. K., Isobe E., Onorato L. Bacteriophage T4 prehead proteinase. I. Purification and properties of a bacteriophage enzyme which cleaves the capsid precursor proteins. J Mol Biol. 1976 Oct 15;107(1):35–54. doi: 10.1016/s0022-2836(76)80016-2. [DOI] [PubMed] [Google Scholar]
  27. Showe M. K., Isobe E., Onorato L. Bacteriophage T4 prehead proteinase. II. Its cleavage from the product of gene 21 and regulation in phage-infected cells. J Mol Biol. 1976 Oct 15;107(1):55–69. doi: 10.1016/s0022-2836(76)80017-4. [DOI] [PubMed] [Google Scholar]
  28. Steven A. C., Aebi U., Showe M. K. Folding and capsomere morphology of the P23 surface shell of bacteriophage T4 polyheads from mutants in five different head genes. J Mol Biol. 1976 Apr 15;102(3):373–400. [PubMed] [Google Scholar]
  29. Steven A. C., Couture E., Aebi U., Showe M. K. Structure of T4 polyheads. II. A pathway of polyhead transformation as a model for T4 capsid maturation. J Mol Biol. 1976 Sep 5;106(1):187–221. doi: 10.1016/0022-2836(76)90307-7. [DOI] [PubMed] [Google Scholar]
  30. Stone K. R., Cummings D. J. Isolation and characterization of two basic internal proteins from the T-even bacteriophages. J Virol. 1970 Oct;6(4):445–454. doi: 10.1128/jvi.6.4.445-454.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Valentine R. C., Pereira H. G. Antigens and structure of the adenovirus. J Mol Biol. 1965 Aug;13(1):13–20. doi: 10.1016/s0022-2836(65)80076-6. [DOI] [PubMed] [Google Scholar]

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