Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Virology logoLink to Journal of Virology
. 1977 Oct;24(1):401–405. doi: 10.1128/jvi.24.1.401-405.1977

Phosphorylated protein component present in influenza virions.

M L Privalsky, E E Penhoet
PMCID: PMC515941  PMID: 904030

Abstract

The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein is in the form of a phosphomonoester. Acid hydrolysis of the isolated nucleoprotein followed by thin-layer electrophoresis identified the phosphorylated amino acid residue as phosphoserine.

Full text

PDF
401

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Roy P., Bean W. J., Jr, Simpson R. W. Transcription of the influenza ribonucleic acid genome by a virion polymerase. 3. Completeness of the transcription process. J Virol. 1972 Oct;10(4):689–697. doi: 10.1128/jvi.10.4.689-697.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Downer D. N., Rogers H. W., Randall C. C. Endogenous protein kinase and phosphate acceptor proteins in vaccinia virus. Virology. 1973 Mar;52(1):13–21. doi: 10.1016/0042-6822(73)90393-0. [DOI] [PubMed] [Google Scholar]
  3. HEPPEL L. A., HARKNESS D. R., HILMOE R. J. A study of the substrate specificity and other properties of the alkaline phosphatase of Escherichia coli. J Biol Chem. 1962 Mar;237:841–846. [PubMed] [Google Scholar]
  4. Hatanaka M., Twiddy E., Gilden R. V. Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology. 1972 Feb;47(2):536–538. doi: 10.1016/0042-6822(72)90297-8. [DOI] [PubMed] [Google Scholar]
  5. Hohmann P., Tobey R. A., Gurley L. R. Phosphorylation of distinct regions of f1 histone. Relationship to the cell cycle. J Biol Chem. 1976 Jun 25;251(12):3685–3692. [PubMed] [Google Scholar]
  6. Paoletti E., Moss B. Protein kinase and specific phosphate acceptor proteins associated with vaccinia virus cores. J Virol. 1972 Sep;10(3):417–424. doi: 10.1128/jvi.10.3.417-424.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosemond H., Moss B. Phosphoprotein component of vaccinia virions. J Virol. 1973 Jun;11(6):961–970. doi: 10.1128/jvi.11.6.961-970.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rubenstein A. S., Gravell M., Darlington R. Protein kinase in enveloped herpes simplex virions. Virology. 1972 Oct;50(1):287–290. doi: 10.1016/0042-6822(72)90374-1. [DOI] [PubMed] [Google Scholar]
  9. Russell W. C., Shekel J. J., Machado R., Pereira H. G. Phosphorylated polypeptides in adenovirus-infected cells. Virology. 1972 Dec;50(3):931–934. doi: 10.1016/0042-6822(72)90450-3. [DOI] [PubMed] [Google Scholar]
  10. Silberstein H., August J. T. Phosphorylation of animal virus proteins by a virion protein kinase. J Virol. 1973 Sep;12(3):511–522. doi: 10.1128/jvi.12.3.511-522.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sokol F., Clark H. F. Phosphoproteins, structural components of rhabdoviruses. Virology. 1973 Mar;52(1):246–263. doi: 10.1016/0042-6822(73)90413-3. [DOI] [PubMed] [Google Scholar]
  12. Strand M., August J. T. Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus. Nat New Biol. 1971 Sep 29;233(39):137–140. doi: 10.1038/newbio233137a0. [DOI] [PubMed] [Google Scholar]
  13. Tan K. B., Sokol F. Structural proteins of simian virus 40: phosphoproteins. J Virol. 1972 Nov;10(5):985–994. doi: 10.1128/jvi.10.5.985-994.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tekamp P., Penhoet E. E. Message activity of influenza viral RNA. J Virol. 1976 May;18(2):812–816. doi: 10.1128/jvi.18.2.812-816.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES