Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Nov;24(2):590–601. doi: 10.1128/jvi.24.2.590-601.1977

Changes in the capsid structure and stability of defective particles of bacteriophage R17.

W J Iglewski
PMCID: PMC515970  PMID: 410949

Abstract

Serological and chemical methods were used to compare the capsid structure and stability of R17 phage and amA31 defective particles. Immunodiffusion analysis demonstrated identity between intact R17 and amA31 capside and between dissociated subunits of both R17 and amA31 and purified coat protein. Radioimmunoassays detected an antibody in R17 antisera that binds to intact R17 but could not be absorbed from R17 antisera with amA31. The R17 antibody remaining in amA31-absorbed sera did not neutralize infectivity of R17 phage. Differences between the surface composition of R17 and amA31 capsids were also detected by iodination. Capsids of R17 bound approximately four times more 125I than amA31, which was accounted for by a decreased 125I labeling of coat protein. Finally, amA31 capsids dissociated under milder conditions of sodium dodecyl sulfate treatment than R17 capsids. The sodium dodecyl sulfate dissociation of both R17 and amA31 capsids resulted in the formation of a transient 38,000-dalton intermediate, which subsequently dissociated to coat protein monomers. Preparations of dissociated R17 capsids also contained assembly protein was also found in preparations of dissociated amA31 capsids.

Full text

PDF
590

Images in this article

595Image
on p.595
596Image
on p.596
596Image
on p.596

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoi T., Kaesberg P. Read-through proteins of group 4 RNA bacteriophages TW19 and TW28. J Virol. 1976 Oct;20(1):330–333. doi: 10.1128/jvi.20.1.330-333.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Curtiss L. K., Krueger R. G. Localization of coliphage MS2 A-protein. J Virol. 1974 Sep;14(3):503–508. doi: 10.1128/jvi.14.3.503-508.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. David G. S., Reisfeld R. A. Protein iodination with solid state lactoperoxidase. Biochemistry. 1974 Feb 26;13(5):1014–1021. doi: 10.1021/bi00702a028. [DOI] [PubMed] [Google Scholar]
  4. Franklin R. M., Granboulan N. Ultrastructure of Escherichia coli cells infected with bacteriophage R17. J Bacteriol. 1966 Feb;91(2):834–848. doi: 10.1128/jb.91.2.834-848.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GESTELAND R. F., BOEDTKER H. SOME PHYSICAL PROPERTIES OF BACTERIOPHAGE R17 AND ITS RIBONUCLEIC ACID. J Mol Biol. 1964 Apr;8:496–507. doi: 10.1016/s0022-2836(64)80007-3. [DOI] [PubMed] [Google Scholar]
  6. Gibson W. Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology. 1974 Dec;62(2):319–336. doi: 10.1016/0042-6822(74)90395-x. [DOI] [PubMed] [Google Scholar]
  7. Heisenberg M. Formation of defective bacteriophage particles by fr amber mutants. J Mol Biol. 1966 May;17(1):136–144. doi: 10.1016/s0022-2836(66)80100-6. [DOI] [PubMed] [Google Scholar]
  8. Hohn T., Hohn B. Structure and assembly of simple RNA bacteriophages. Adv Virus Res. 1970;16:43–98. doi: 10.1016/s0065-3527(08)60021-4. [DOI] [PubMed] [Google Scholar]
  9. Iglewski W. J., Franklin R. M. Denaturation and renaturation of viral RNA. 3. Purification of the complementary strand of R17 RNA. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1019–1024. doi: 10.1073/pnas.58.3.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iglewski W. J. Transfection: enhancement by assembly protein of bacteriophage R17. Biochem Biophys Res Commun. 1976 Jan 12;68(1):299–306. doi: 10.1016/0006-291x(76)90043-7. [DOI] [PubMed] [Google Scholar]
  11. Jockusch H., Ball L. A., Kaesberg P. Synthesis of polypeptides directed by the RNA of phage Q beta. Virology. 1970 Oct;42(2):401–414. doi: 10.1016/0042-6822(70)90283-7. [DOI] [PubMed] [Google Scholar]
  12. Leipold B., Hofschneider P. H. Isolation of an infectious RNA-A-protein complex from the bacteriophage M12. FEBS Lett. 1975 Jul 15;55(1):50–52. doi: 10.1016/0014-5793(75)80954-9. [DOI] [PubMed] [Google Scholar]
  13. Lodish H. F., Horiuchi K., Zinder N. D. Mutants of the bacteriophage f2. V. On the production of noninfectious phage particles. Virology. 1965 Oct;27(2):139–155. doi: 10.1016/0042-6822(65)90154-6. [DOI] [PubMed] [Google Scholar]
  14. Matsuda M., Kanei C., Yoneda M. Comparison o the peptide "finger print" and antigenic specificity of a diphtheria toxin fragment produced by a phage-mutant lysogen and "fragments A and B" of the toxin. Biken J. 1972 Dec;15(4):199–206. [PubMed] [Google Scholar]
  15. Pons M. W., Hirst G. K. Polyacrylamide gel electrophoresis of influenza virus RNA. Virology. 1968 Feb;34(2):385–388. doi: 10.1016/0042-6822(68)90257-2. [DOI] [PubMed] [Google Scholar]
  16. Roberts J. W., Steitz J. E. The reconstitution of infective bacteriophage R17. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1416–1421. doi: 10.1073/pnas.58.4.1416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rohrmann G. F., Krueger R. G. Physical, biochemical, and immunological properties of coliphage MS-2 particles. J Virol. 1970 Sep;6(3):269–279. doi: 10.1128/jvi.6.3.269-279.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shiba T., Miyake T. New type of infectious complex of E. coli RNA phage. Nature. 1975 Mar 13;254(5496):157–158. doi: 10.1038/254157a0. [DOI] [PubMed] [Google Scholar]
  19. Spangler V. L., Iglewski W. J. Effect of R17 Bacteriophage-Specific Proteins on Ribosomal Ribonucleic Acid Synthesis of Escherichia coli. J Virol. 1972 May;9(5):792–799. doi: 10.1128/jvi.9.5.792-799.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steitz J. A. Identification of the A protein as a structural component of bacteriophage R17. J Mol Biol. 1968 May 14;33(3):923–936. doi: 10.1016/0022-2836(68)90328-8. [DOI] [PubMed] [Google Scholar]
  21. Sugiyama T., Hebert R. R., Hartman K. A. Ribonucleoprotein complexes formed between bacteriophage MS2 RNA and MS2 protein in vitro. J Mol Biol. 1967 May 14;25(3):455–463. doi: 10.1016/0022-2836(67)90198-2. [DOI] [PubMed] [Google Scholar]
  22. Sugiyama T., Nakada D. Translational control of bacteriophage MS2 RNA cistrons by MS2 coat protein: polyacrylamide gel electrophoretic analysis of proteins synthesized in vitro. J Mol Biol. 1968 Feb 14;31(3):431–440. doi: 10.1016/0022-2836(68)90419-1. [DOI] [PubMed] [Google Scholar]
  23. Tooze J., Weber K. Isolation and characterization of amber mutants of bacteriophage R17. J Mol Biol. 1967 Sep 14;28(2):311–330. doi: 10.1016/s0022-2836(67)80012-3. [DOI] [PubMed] [Google Scholar]
  24. Vasquez C., Granboulan N., Franklin R. M. Structure of the ribonucleic acid bacteriophage R17. J Bacteriol. 1966 Dec;92(6):1779–1786. doi: 10.1128/jb.92.6.1779-1786.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weber K. Amino acid sequence studies on the tryptic peptides of the coat protein of the bacteriophage R17. Biochemistry. 1967 Oct;6(10):3144–3154. doi: 10.1021/bi00862a023. [DOI] [PubMed] [Google Scholar]
  26. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  27. Wilheim E., Lamm M. E. Absence of allotype b4 in the heavy chains of rabbit gamma-G-immunoglobulin. Nature. 1966 Nov 19;212(5064):846–847. doi: 10.1038/212846a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES