Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Nov;24(2):651–661. doi: 10.1128/jvi.24.2.651-661.1977

Unique peptide maps of the three largest proteins specified by the flavivirus Kunjin.

P J Wright, D S Bowden, E G Westaway
PMCID: PMC515977  PMID: 916030

Abstract

Tryptic digests of four polypeptides found in Kunjin virus-infected Vero cells, NV5, NV4, V3, and NV3, were compared by peptide mapping. The polypeptides to be analyzed were labeled with radioactive methionine and separated by electrophoresis through polyacrylamide gels containing sodium dodecyl sulfate. Because infection of Vero cells by Kunjin virus does not inhibit host cell protein synthesis, radioactively labeled viral polypeptides prepared from infected cells migrate coincidentally during sodium dodecyl sulfate-gel electrophoresis with some of the labeled host proteins. Thus, the genuine viral methionine-containing peptides in tryptic digests of viral proteins have been identified by co-analyzing polypeptides from [3H]methionine-labeled uninfected cells and [35S]methionine-labeled infected cells and determining the 35S/3H ratio in the peptides resolved in two dimensions on thin-layer chromatography plates. The peptide map of NV3 demonstrated that it is host coded, whereas NV5, NV4, and V3 have unique peptide maps and, therefore, account for approximately one-half of the coding potential of Kunjin virus RNA.

Full text

PDF
651

Images in this article

653Image
on p.653
653Image
on p.653
654Image
on p.654
655Image
on p.655
657Image
on p.657
658Image
on p.658
659Image
on p.659

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulton R. W., Westaway E. G. Comparisons of togaviruses: Sindbis virus (group A) and Kunjin virus (group B). Virology. 1972 Jul;49(1):283–289. doi: 10.1016/s0042-6822(72)80029-1. [DOI] [PubMed] [Google Scholar]
  2. Boulton R. W., Westaway E. G. Replication of the flavivirus Kunjin: proteins, glycoproteins, and maturation associated with cell membranes. Virology. 1976 Feb;69(2):416–430. doi: 10.1016/0042-6822(76)90473-6. [DOI] [PubMed] [Google Scholar]
  3. Burke D. J., Keegstra K. Purification and composition of the proteins from Sindbis virus grown in chick and BHK cells. J Virol. 1976 Dec;20(3):676–686. doi: 10.1128/jvi.20.3.676-686.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clewley J. P., Kennedy S. I. Purification and polypeptide composition of Semliki Forest virus RNA polymerase. J Gen Virol. 1976 Sep;32(3):395–411. doi: 10.1099/0022-1317-32-3-395. [DOI] [PubMed] [Google Scholar]
  5. Hindennach I., Jockusch H. Peptide mapping of phage Q beta proteins using cell-free synthesis. Virology. 1974 Aug;60(2):327–341. doi: 10.1016/0042-6822(74)90328-6. [DOI] [PubMed] [Google Scholar]
  6. Kennedy S. I., Burke D. C. Studies on the structural proteins of Semliki Forest virus. J Gen Virol. 1972 Jan;14(1):87–98. doi: 10.1099/0022-1317-14-1-87. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Pinter A., Compans R. W. Sulfated components of enveloped viruses. J Virol. 1975 Oct;16(4):859–866. doi: 10.1128/jvi.16.4.859-866.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Qureshi A. A., Trent D. W. Group B arbovirus structural and nonstructural antigens. 3. Serological specificity of solubilized intracellular viral proteins. Infect Immun. 1973 Dec;8(6):993–999. doi: 10.1128/iai.8.6.993-999.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. The proteins of Japanese encephalitis virus. Virology. 1971 Apr;44(1):108–124. doi: 10.1016/0042-6822(71)90158-9. [DOI] [PubMed] [Google Scholar]
  11. Shapiro D., Brandt W. E., Russell P. K. Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses. Virology. 1972 Dec;50(3):906–911. doi: 10.1016/0042-6822(72)90445-x. [DOI] [PubMed] [Google Scholar]
  12. Shapiro D., Kos K. A., Russel P. K. Japanese encephalitis virus glycoproteins. Virology. 1973 Nov;56(1):88–94. doi: 10.1016/0042-6822(73)90289-4. [DOI] [PubMed] [Google Scholar]
  13. Shapiro D., Kos K. A., Russell P. K. Protein synthesis in Japanese encephalitis virus-infected cells. Virology. 1973 Nov;56(1):95–109. doi: 10.1016/0042-6822(73)90290-0. [DOI] [PubMed] [Google Scholar]
  14. Shapiro D., Kos K., Brandt W. E., Russell P. K. Membrane-bound proteins of Japanese encephalitis virus-infected chick embryo cells. Virology. 1972 May;48(2):360–372. doi: 10.1016/0042-6822(72)90046-3. [DOI] [PubMed] [Google Scholar]
  15. Stollar V. Studies on the nature of dengue viruses. IV. The structural proteins of type 2 dengue virus. Virology. 1969 Nov;39(3):426–438. doi: 10.1016/0042-6822(69)90091-9. [DOI] [PubMed] [Google Scholar]
  16. Tan K. B., Sokol F. Phosphorylation of simian virus 40 proteins in a cell-free system. J Virol. 1973 Oct;12(4):696–703. doi: 10.1128/jvi.12.4.696-703.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Trent D. W., Swensen C. C., Qureshi A. A. Synthesis of Saint Louis encephalitis virus ribonucleic acid in BHK-21-13 cells. J Virol. 1969 Apr;3(4):385–394. doi: 10.1128/jvi.3.4.385-394.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Westaway E. G., McKimm J. L., McLeod L. G. Heterogeneity among flavivirus proteins separated in slab gels. Arch Virol. 1977;53(4):305–312. doi: 10.1007/BF01315629. [DOI] [PubMed] [Google Scholar]
  19. Westaway E. G. Proteins specified by group B togaviruses in mammalian cells during productive infections. Virology. 1973 Feb;51(2):454–465. doi: 10.1016/0042-6822(73)90444-3. [DOI] [PubMed] [Google Scholar]
  20. Westaway E. G., Reedman B. M. Proteins of the group B arbovirus Kunjin. J Virol. 1969 Nov;4(5):688–693. doi: 10.1128/jvi.4.5.688-693.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Westaway E. G., Shew M. Proteins and glycoproteins specified by the flavivirus Kunjin. Virology. 1977 Jul 15;80(2):309–319. doi: 10.1016/s0042-6822(77)80007-x. [DOI] [PubMed] [Google Scholar]
  22. Westaway E. G. Strategy of the flavivirus genome: evidence for multiple internal initiation of translation of proteins specified by Kunjin virus in mammalian cells. Virology. 1977 Jul 15;80(2):320–335. doi: 10.1016/s0042-6822(77)80008-1. [DOI] [PubMed] [Google Scholar]
  23. Westaway E. G. The proteins of Murray Valley encephalitis virus. J Gen Virol. 1975 Jun;27(3):293–292. doi: 10.1099/0022-1317-27-3-293. [DOI] [PubMed] [Google Scholar]
  24. Wright P. J., Di Mayorca G. Virion polypeptide composition of the human papovavirus BK: comparison with simian virus 40 and polyoma virus. J Virol. 1975 Apr;15(4):828–835. doi: 10.1128/jvi.15.4.828-835.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wright P. J., Westaway E. G. Comparisons of the peptide maps of Kunjin virus proteins smaller than the envelope protein. J Virol. 1977 Nov;24(2):662–672. doi: 10.1128/jvi.24.2.662-672.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES