Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1977 Nov;24(2):701–703. doi: 10.1128/jvi.24.2.701-703.1977

BALB/3T3 cells infected by the ts3 mutant of polyoma virus fail to accumulate virus-specific early RNA at the nonpermissive temperature.

B Cogen, W Eckhart
PMCID: PMC515983  PMID: 199746

Abstract

We measured the accumulation of virus-specific early RNA in BALB/3T3 cells infected by the ts3 mutant of polyoma virus by annealing cytoplasmic RNA from infected cells to purified, radiolabeled, "early" strand of polyoma DNA. Cells infected by the ts3 mutant fail to accumulate virus-specific early RNA at the nonpermissive temperature.

Full text

PDF
701

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avila J., Saral R., Martin R. G., Khoury G. The temperature-sensitive defect in SV40 group D mutants. Virology. 1976 Aug;73(1):89–95. doi: 10.1016/0042-6822(76)90063-5. [DOI] [PubMed] [Google Scholar]
  2. Bacheler L. T. Virus-secific transcription in 3T3 cells transformed by the ts-a mutant of polyoma virus. J Virol. 1977 Apr;22(1):54–64. doi: 10.1128/jvi.22.1.54-64.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eckhart W. Complementation between temperature-sensitive (ts) and host range nontransforming (hr-t) mutants of polyoma virus. Virology. 1977 Apr;77(2):589–597. doi: 10.1016/0042-6822(77)90484-6. [DOI] [PubMed] [Google Scholar]
  4. Eckhart W., Dulbecco R. Properties of the ts3 mutant of polyoma virus during lytic infection. Virology. 1974 Aug;60(2):359–369. doi: 10.1016/0042-6822(74)90331-6. [DOI] [PubMed] [Google Scholar]
  5. Feunteun J., Sompayrac L., Fluck M., Benjamin T. Localization of gene functions in polyoma virus DNA. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4169–4173. doi: 10.1073/pnas.73.11.4169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frost E., Bourgaux-Ramoisy D., Bourgaux P. Short communications Decapsidation of polyoma virus mutants. Virology. 1975 Nov;68(1):256–259. doi: 10.1016/0042-6822(75)90166-x. [DOI] [PubMed] [Google Scholar]
  7. Gibson W., Hunter T., Cogen B., Eckhart W. Altered virion proteins of a temperature-sensitive mutant of polyoma virus, ts59. Virology. 1977 Jul 1;80(1):21–41. doi: 10.1016/0042-6822(77)90378-6. [DOI] [PubMed] [Google Scholar]
  8. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  9. Lai C. J., Nathans D. Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology. 1974 Aug;60(2):466–475. doi: 10.1016/0042-6822(74)90340-7. [DOI] [PubMed] [Google Scholar]
  10. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  11. Robb J. A., López-Revilla R. Temperature-dependent synthesis of early and late SV40 cytoplasmic RNA during tsD101 mutant virion infection. Intervirology. 1975;6(2):122–128. doi: 10.1159/000149464. [DOI] [PubMed] [Google Scholar]
  12. Robb J. A., Martin R. G. Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. J Virol. 1972 Jun;9(6):956–968. doi: 10.1128/jvi.9.6.956-968.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES