Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Dec;24(3):826–835. doi: 10.1128/jvi.24.3.826-835.1977

Transcriptase activity associated with rabies virion.

A Kawai
PMCID: PMC516004  PMID: 22766

Abstract

Rabies virion-associated transcriptase activity was investigated in vitro and compared with that of the New Jersey serotype of vesicular stomatitis virus. The concentration of detergent that affected [3H]GMP incoporation into acid-insoluble material was significantly different for both viruses. Vesicular stomatitis virus New Jersey required 0.05 to 0.1% nonionic detergent, whereas rabies virion could not be fully activated unless 4 to 5% detergent was used. Other optimal conditions were as follows: 40 mM NaCl, 5 mM Mg2+, 40 mM Tris-hydrochloride (pH 7.4), 5 mM dithiothreitol, and 30 degrees C. The reaction required four nucleoside triphosphates. The initial rate of RNA synthesis by rabies virion enzyme was 140 pmol of GMP incorporated/mg of viral protein per h and linearly increased until about 8 h, with a slight initial lag phase. The enzyme activity that correlated with the content of L protein was highest when rabies virions were grown at 33 degrees C. The product was single-stranded RNA, which was complementary in base sequences to rabies viral RNA. Most of the RNA synthesized sedimented at 6-16S.

Full text

PDF
826

Images in this article

832Image
on p.832
834Image
on p.834

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaslestad H. G., Clark H. F., Bishop D. H., Koprowski H. Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. J Virol. 1971 Jun;7(6):726–735. doi: 10.1128/jvi.7.6.726-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernard J. P., Northrop R. L. RNA polymerase in mumps virion. J Virol. 1974 Jul;14(1):183–186. doi: 10.1128/jvi.14.1.183-186.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang S. H., Hefti E., Obijeski J. F., Bishop D. H. RNA transcription by the virion polymerases of five rhabdoviruses. J Virol. 1974 Mar;13(3):652–661. doi: 10.1128/jvi.13.3.652-661.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark H. F., Wiktor T. J. Temperature-sensitivity characteristics distinguishing substrains of fixed rabies virus: lack of correlation with plague-size markers or virulence for mice. J Infect Dis. 1972 Jun;125(6):637–646. doi: 10.1093/infdis/125.6.637. [DOI] [PubMed] [Google Scholar]
  7. Emerson S. U., Wagner R. R. L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1973 Dec;12(6):1325–1335. doi: 10.1128/jvi.12.6.1325-1335.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kawai A., Matsumoto S., Tanabe K. Characterization of rabies viruses recovered from persistently infected BHK cells. Virology. 1975 Oct;67(2):520–533. doi: 10.1016/0042-6822(75)90452-3. [DOI] [PubMed] [Google Scholar]
  10. Knudson D. L. Rhabdoviruses. J Gen Virol. 1973 Jun;20(Suppl):105–130. doi: 10.1099/0022-1317-20-Supplement-105. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Marx P. A., Portner A., Kingsbury D. W. Sendai virion transcriptase complex: polyeptide composition and inhibition by virion envelope proteins. J Virol. 1974 Jan;13(1):107–112. doi: 10.1128/jvi.13.1.107-112.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McSharry J., Benzinger R. Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation". Virology. 1970 Mar;40(3):745–746. doi: 10.1016/0042-6822(70)90219-9. [DOI] [PubMed] [Google Scholar]
  15. Naito S., Ishihama A. Function and structure of RNA polymerase from vesicular stomatitis virus. J Biol Chem. 1976 Jul 25;251(14):4307–4314. [PubMed] [Google Scholar]
  16. Robinson W. S. Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid. J Virol. 1971 Jul;8(1):81–86. doi: 10.1128/jvi.8.1.81-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schneider L. G., Diringer H. Structure and molecular biology of rabies virus. Curr Top Microbiol Immunol. 1976;75:153–180. doi: 10.1007/978-3-642-66530-1_5. [DOI] [PubMed] [Google Scholar]
  18. Sedwick W. D., Wiktor T. J. Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. J Virol. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sokol F., Clark H. F. Phosphoproteins, structural components of rhabdoviruses. Virology. 1973 Mar;52(1):246–263. doi: 10.1016/0042-6822(73)90413-3. [DOI] [PubMed] [Google Scholar]
  20. Stone H. O., Portner A., Kingsbury D. W. Ribonucleic acid transcriptases in Sendai Virions and infected cells. J Virol. 1971 Aug;8(2):174–180. doi: 10.1128/jvi.8.2.174-180.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Villarreal L. P., Holland J. J. Transcribing complexes in cells infected by vesicular stomatitis virus and rabies virus. J Virol. 1974 Sep;14(3):441–450. doi: 10.1128/jvi.14.3.441-450.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., MacLeod R. Classification of rhabdovirus proteins: a proposal. J Virol. 1972 Dec;10(6):1228–1230. doi: 10.1128/jvi.10.6.1228-1230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES