Table 1. Cell cycle timing in daughter cells.
Strain | T0 (min) | T1 (min) | T2 (min) | S/G2/M (min) |
---|---|---|---|---|
Wild type | 6 ± 2 (50) | 19 ± 10 (50) | 17 ± 9 (50) | 53 ± 8 (50) |
CLB3∆db | 6 ± 2 (50) | 0 ± 1 (50) | 18 ± 10 (50) | 71 ± 16 (50) |
Times are mean ± SD. Number of cells scored in parentheses. We define T0 as the time from Whi5 entry to cytokinesis (Myo1 ring disappearance) (Figure 2); T1, time from Myo1 ring disappearance to Whi5 exit; T2, time from Whi5 exit to bud emergence (Di Talia et al. 2007); S/G2/M, time from Myo1 ring appearance at incipient bud neck to disappearance at cytokinesis. The frame resolution was 3 min.