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. 2016 Dec 1;10(6):466–483. doi: 10.1080/19336896.2016.1254857

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© 2016 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — LPS-induced conversion of truncated recPrP generates large oligomers (LO) and small oligomers (9-mer to 20-mers). (A) Concentration dependent conversion of 1 mg/mL recShPrP^90–232 in 15 mM NaPO₄, 3.3 mM NaCH₃CO₂ at pH 6.5 with 0.005 mg/mL LPS (lane 1), 0.01 mg/mL LPS (lane 2), 0.09 mg/mL (lane 3), 0.17 mg/mL (lane 4) and 0.75 mg/mL LPS (lane 5). The formation of small oligomers sized 9-mer to 20-mer of 0.5 mg/mL recShPrP^90–232 in 150 mM NaCl (unbuffered, pH 5.5) with 0.5 mg/mL LPS (lane 6) was sized by the known size of oligomers generated by shaking at 350 rpm with (lane 7) and without (lane 8) LPS. (B) LPS converts and generates different amounts of small oligomers with 1 mg/mL recShPrP^90–232 (lane 1), recMoPrP^90–231 (lane 2), and recCePrP^94–233 (lane 3) with 0.75 mg/mL LPS in buffer (15 mM NaPO₄, 3.3 mM NaCH₃CO₂ pH 6.5). Lane 4 is monomeric recShPrP^90–232. Monomer is labeled with M and dimer is labeled as D.