Skip to main content
. 2016 Oct 3;10(6):444–465. doi: 10.1080/19336896.2016.1234574

FIGURE 6.

FIGURE 6.

Hsp31 and Hsp104 reduce Sup35 prion toxicity. Hsp31, Hsp104 or the indicated combination of both along with GAL-PrD-Sup35-EYFP or full length Sup35 were overexpressed in [PSI+] and [PSI+] hsp31Δ strains. Decimal serial dilutions were plated onto selection plates with 2% glucose that serve as control or 2% galactose to induce the expression. Plates were incubated at 30°C for 3 d before producing the images. Hsp31 or Hsp104 rescued toxicity of GAL-PrD-Sup35-EYFP or full length Sup35 in these strains. Combination of Hsp31 and Hsp104 greatly reduce the toxicity compared to when they are individually expressed.