Yanhao Chen, Xiaojian Liu, Yongxian Zhang, Hui Wang, Hao Ying, Mingyao Liu, Dali Li, Kathy O Lui and Qiurong Ding
Molecular Therapy (2016) 24: 1508–1510. doi: 10.1038/mt.2016.172
The authors inadvertently omitted to include in the reference list a previous publication by Richard Moore and colleagues describing a similar approach to controlling the copies and residence time of an exogenous gene product by introducing CRISPR/Cas9-mediated cleavage of the delivery vector, thereby inactivating a co-expressed gene of interest. The article should been included as reference 12 and cited where strategies for controlling gene expression are discussed. For example, a sentence describing Moore and coauthors' work should have been inserted after the second to last sentence as follows: “Moreover, the self-restricted CRISPR system can also be adopted to other viral vectors including AdVs and AAVs, and can be used in combination with currently available strategies to reduce the off-target effects including the use of Cas9 nickases,8 Cas9-FokI chimeric proteins,9 or proper modifications to residues of the Cas9 protein.10,11 A similar methodology, as reported by Moore et al.,12 can be applied to control the copies and residence time of any exogenous gene product through CRISPR/Cas9-mediated cleavage of the delivery vector, thereby inactivating a co-expressed gene of interest.”
12. Moore, R, Spinhirne, A, Lai, MJ, Preisser, S, Li, Y, Kang, T et al. (2015). CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res 43:1297–1303.