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. 2016 Oct 20;12(12):2386–2403. doi: 10.1080/15548627.2016.1240856

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Figure 3. — LC3B and LC3B^GΔ insertion into lipid monolayers. (A) Representative time courses of LC3B adsorption at the air-water interface (left-hand panel) and representative time courses of increase in lateral pressure after LC3B (1.5 μM) insertion into PC:DOPE (80:20 mol ratio) and PC:DOPE:CL (55:20:25 mol ratio) monolayers (right-hand panel). (B) Maximum increase in lateral pressure after LC3B and LC3B^GΔ insertion into lipid monolayers. Lipids were: [•] PC:DOPE (80:20 mol ratio), [▴] PC:DOPE:CL, [○] PC:DOPE:PS and [Δ] PC:DOPE:PtdIns4P (55:20:25 mol ratio). Data reported as a function of initial lateral pressure π₀. Very similar results were obtained at 37°C, data not shown.