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. 2016 May 5;7(27):41432–41444. doi: 10.18632/oncotarget.9180

Figure 6. Andrographolide induced apoptosis signaling is dependent on IRE-1.

Figure 6

A. T84 cells were transfected with plasmid for overexpression of IRE-1 and then treated with Andrographolide. Cell lysates were analyzed by western blot and quantified by densitometry for expression of IRE-1, CHOP, and Bcl-2. Expression is normalized against GAPDH expression. T84 cells were transfected with siRNA for IRE-1 or control siRNA and treated with Andrographolide for 48 h and B. DNA fragmentation was compared using a colorimetric assay. (C) Cells were also evaluated for mRNA expression by qRT-PCR for C. IRE-1, D. CHOP, E. Bcl-2, and F. BAX. (***P < 0.001)