Skip to main content
. 2016 May 18;7(27):41445–41459. doi: 10.18632/oncotarget.9441

Figure 6. Rho/ROCK promotes IL-6 transcription via activation of IKKβ/NF-κB.

Figure 6

(A) Proteasome inhibitor MG132 was added to inhibit the degradation of p-IκBα. p-IκBα was reduced by 24-hour treatment of 10 μM Y27632 in BEL-7402 and SMMC-7721 cells. (B) BEL-7402 and SMMC-7721 cells were treated with indicated concentrations of Y27632 for 3 hours. Phosphorylation of IKKβ was suppressed by Y27632 treatment. (C) Inhibition of ROCK by Y27632 and ROCK2 knockdown both repressed NF-κB-driven transcription of firefly luciferase. SV40-driven expression of Renilla luciferase was used as normalization control. (D) BEL-7402 and SMMC-7721 cells were treated with the indicated concentration of NF-κB inhibitor, BAY11-7082, for 4 hours. mRNA expression level of IL-6 was decreased by BAY11-7082 in a dose-dependent manner. In all panels, 3 experiments are represented. P-value was calculated using Student's t test.