FIG. 6.

Pharmacological inhibition of ERK1/2 or Akt blocks the synergistic induction of NIS by TSH + IGF-1. Human thyrocytes were pretreated with the MEK1/2 (ERK1/2) inhibitor U0126 (final concentration 1 μM) or the Akt inhibitor MK-2206 (final concentration 5 μM) for 30 min, then treated with the indicated dose of TSH alone or in combination with 100 ng/mL of IGF-1. RNA was isolated after 48 hours, cDNA synthesized, and the indicated genes measured by quantitative PCR. Data are expressed as mean ± SEM, n = 4.