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Infection and Immunity logoLink to Infection and Immunity
. 2004 Oct;72(10):6190. doi: 10.1128/IAI.72.10.6190.2004

Genetic Evidence that Legionella pneumophila RpoS Modulates Expression of the Transmission Phenotype in Both the Exponential Phase and the Stationary Phase

Michael A Bachman 1, Michele S Swanson 1
PMCID: PMC517605

Volume 72, no. 5, p. 2468-2476, 2004. Pages 2468-2474: “mip” should read “magA.” The mip nucleotide sequence file (GenBank accession number S71704), used to design probes for Northern analysis (Fig. 1), was mislabeled in GenBank and instead describes the gene magA (P. Hoffman, personal communication). As recently reported (M. F. Hiltz, G. R. Sisson, A. K. C. Brassinga, E. Garduno, R. A. Garduno, and P. S. Hoffman, J. Bacteriol. 186:3038-3045, 2004), this error, which has now been corrected, led to incorrect assignment of Mip homology to MagA homologues in other bacterial species. MagA is a 20-kDa protein containing a conserved motif of AhpD-type reductases, encoded within the 65-kb pathogenicity island of Legionella pneumophila Philadelphia-1 strains and highly expressed in the mature intracellular form of L. pneumophila (R. A. Garduno, E. Garduno, M. Hiltz, and P. S. Hoffman, Infect. Immun. 70:6273-6283, 2002; A. K. C. Brassinga, M. F. Hiltz, G. R. Sisson, M. G. Morash, N. Hill, E. Garduno, P. H. Edelstein, R. A. Garduno, and P. S. Hoffman, J. Bacteriol. 185:4630-4637, 2003; and M. F. Hiltz, G. R. Sisson, A. K. C. Brassinga, E. Garduno, R. A. Garduno, and P. S. Hoffman, J. Bacteriol. 186:3038-3045, 2004). Consistent with the presence of RhoS-specific promoter sequences upstream of magA and Western analysis (M. F. Hiltz, G. R. Sisson, A. K. C. Brassinga, E. Garduno, R. A. Garduno, and P. S. Hoffman, J. Bacteriol. 186:3038-3045, 2004), RpoS and, to a lesser extent, LetA/LetS and LetE induce expression of magA in the stationary phase. We did not analyze expression of mip, encoding the macrophage infectivity potentiator. The results of the paper are not affected by this correction.


Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

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