TABLE I.
Summary of the DNA and molecular beacons sequences. Mutated DNA: underlined; Pull-down I region: bold; Pull-down II region: italic; and Molecular beacon region: gray shading.
| Name | Sequence (5′-3′) | Length |
|---|---|---|
| BRAF DNA | CTACACCTCAGATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGAGAAATCTCGATGGAGTGGGTCCCATCAGTTT | 100 |
| BRAF pull-down I | AGATTTCTCTGTAG | 14 |
| BRAF pull-down II | CACCTATTTTTACTGTGAGGTCTTCATGAAGAAATATATCTGAGG | 45 |
| BRAF beacon | TYE665dye-cgcgcaACCCACTCCATCGAGATTTCTCTGTAGCTAGACCAtgcgcg-Quencher | 35/6stem |
| NRAS DNA | CTTACAGAAAACAAGTGGTTATAGATGGTGAAACCTGTTTGTTGGACATACTGGATACAGCTGGACGAGAAGAGTACAGTGCCATGAGAGACCAATACAT | 100 |
| NRAS pull-down I | TCTTCTCGTCCA | 12 |
| NRAS pull-down II | TATGTCCAACAAACAGGTTTCACCATCTATAACCACTTGTTTTCT | 45 |
| NRAS beacon | IRdye750-ggcggTCTTCTCGTCCAGccgcc-IRQuencher | 13/5stem |